Positive control:
Lane 1: Jurkat cell lysate
Lane 2: Hela cell lysate
Applications
-
WB
-
ICC
-
IF
-
IHC-P
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
CD147 Recombinant Rabbit Monoclonal Antibody [JF1-045] (ET1702-58)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
Jurkat cell lysate, Hela cell lysate, Hela, A431, SKOV-3, human colon carcinoma tissue, mouse brain tissue, mouse heart tissue, rat brain tissue, mouse testis tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JF1-045
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
42 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:2,000
-
ICC/IF
-
1:50-1:200
-
IHC-P
-
1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
CD147
SYNONYMS
5A11 antigen antibody; 5F7 antibody; BASI_HUMAN antibody; Basigin (Ok blood group) antibody; Basigin antibody; Blood brain barrier HT7 antigen antibody; Bsg antibody; CD 147 antibody; CD147 antibody; CD147 antigen antibody; Collagenase stimulatory factor antibody; EMMPRIN antibody; Extracellular matrix metalloproteinase inducer antibody; Leukocyte activation antigen M6 antibody; M 6 antibody; M6 antibody; M6 leukocyte activation antigen antibody; Neurothelin antibody; OK antibody; OK blood group antibody; OK blood group antigen antibody; TCSF antibody; Tumor cell derived collagenase stimulatory factor antibody; Tumor cell-derived collagenase stimulatory factor antibody
TISSUE SPECIFICITY
Expressed in erythrocytes (at protein level). Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.
POST-TRANSLATIONAL MODIFICATION
N-glycosylated.
SUBCELLULAR LOCATION
Cell membrane, Melanosome.
FUNCTION
Extracellular matrix metalloproteinase inducer (EMMPRIN), also designated basigin or CD147, is involved in the regulation of matrix remodeling at the epidermal-dermal interface. EMMPRIN stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes, which are typically increased during tissue degradation and wound healing, are important factors in cancer invasion and metastasis.