CD146 Recombinant Rabbit Monoclonal Antibody [JB42-35] (ET7107-91)
Catalog# ET7107-91
Rabbit Monoclonal to CD146
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Rat
Lane 1: A375 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: HUVEC cell lysate (20 µg/Lane)
Lane 4: HepG2 cell lysate (20 µg/Lane)
Lane 5: Mouse lung tissue lysate (40 µg/Lane)
Predicted band size: 72 kDa
Observed band size: 120 kDa
Exposure time: 14 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-91) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
CD146 Recombinant Rabbit Monoclonal Antibody [JB42-35] (ET7107-91)
Immunogen
Synthetic peptide within c-terminal human cd146 (cytoplasmic).
Host
Rabbit
Positive Control
A375 cell lysate, HeLa cell lysate, HUVEC cell lysate, HepG2 cell lysate, Mouse lung tissue lysate, A375, human tonsil tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JB42-35
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:100
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IHC-P
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1:200
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 72 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
CD146
SYNONYMS
A32 antigen antibody;CD 146 antibody;CD146 antibody;CD146 antigen antibody;Cell surface glycoprotein MUC18 antibody;Cell surface glycoprotein P1H12 antibody;Gicerin antibody;Mcam antibody;Melanoma adhesion molecule antibody;Melanoma associated antigen A32 antibody;Melanoma associated antigen MUC18 antibody;Melanoma associated glycoprotein MUC18 antibody;Melanoma cell adhesion molecule antibody;Melanoma-associated antigen A32 antibody;Melanoma-associated antigen MUC18 antibody;MelCAM antibody;MUC 18 antibody;MUC18 antibody;MUC18_HUMAN antibody;S endo 1 antibody;S endo 1 endothelial associated antigen antibody;S-endo 1 endothelial-associated antigen antibody
TISSUE SPECIFICITY
Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis.
SUBCELLULAR LOCATION
Membrane.
FUNCTION
The tumorigenic and metastatic phenotype of melanoma cells correlates well with an increased expression of cell-cell and cell-matrix adhesion receptors. The human Mel-CAM gene encodes a transmembrane glycoprotein, also designated MCAM, MUC18 or CD146, that belongs to the immunoglobulin superfamily and functions as a Ca2+-independent cell adhesion molecule. The deduced human sequence of 603 amino acids consists of a signal peptide, five immunoglobulin-like domains, a transmembrane region and a short cytoplasmic tail. Mel-CAM expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. Mel-CAM is found on 80% of advanced primary human mela-nomas and correlates well with development of metastatic disease. Mel-CAM activation initiates an outside-in signaling pathway that involves the protein tyrosine kinases Fyn, FAK and paxillin. Mel-CAM influences the dynamics of Actin cytoskeleton rearrangement and is essential for the maintenance of thymic architecture and function.
CITATIONS
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Ming Zhang,Kangshou Yao
Proteomic Analyses Reveal Common Promiscuous Patterns of Cell Surface Proteins on Human Embryonic Stem Cells and Sperms
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ICC
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Human
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Mouse
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Citation
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