CD133 Mouse Monoclonal Antibody [A8C2] (HA601025)

UNIPROT #

O43490

PROTEIN NAME

CD133

SYNONYMS

AC133 antibody;Antigen AC133 antibody;CD133 antibody;CORD12 antibody;Hematopoietic stem cell antigen antibody;hProminin antibody;MCDR2 antibody;MSTP061 antibody;OTTHUMP00000217744 antibody;OTTHUMP00000217745 antibody;OTTHUMP00000217746 antibody;PROM1 antibody;PROM1_HUMAN antibody;Prominin I antibody;Prominin like 1 antibody;Prominin like protein 1 precursor antibody;Prominin mouse like 1 antibody;Prominin-1 antibody;Prominin-like protein 1 antibody;Prominin1 antibody;PROML1 antibody;RP41 antibody;STGD4 antibody

SUBCELLULAR LOCATION

Endoplasmic reticulum. Plasma membrane. Cell projection.

FUNCTION

CD133 antigen, also known as prominin-1, is a glycoprotein that in humans is encoded by the PROM1 gene. It is a member of pentaspan transmembrane glycoproteins, which specifically localize to cellular protrusions. When embedded in the cell membrane, the membrane topology of prominin-1 is such that the N-terminus extends into the extracellular space and the C-terminus resides in the intracellular compartment. The protein consists of five transmembrane segments, with the first and second segments and the third and fourth segments connected by intracellular loops while the second and third as well as fourth and fifth transmembrane segments are connected by extracellular loops. CD133 is the most commonly used marker for isolation of cancer stem cell (CSC) population from different tumors, mainly from various gliomas and carcinomas. CD133+ melanoma cells are considered a subpopulation of CSC and play a critical role in recurrence. Moreover, CD133+ melanoma cells are immunogenic and can be used as an antimelanoma vaccination. In mice the vaccination with CD133+ melanoma cells mediated strong anti-tumor activity that resulted in the eradication of parental melanoma cells. In addition, it has also been shown that CD133+ melanoma cells preferentially express the RNA helicase DDX3X . As DDX3X also is an immunogenic protein, the same anti-melanoma vaccination strategy can be employed to give therapeutic antitumor immunity in mice.