CD13 Recombinant Rabbit Monoclonal Antibody [SN71-04] (ET1611-61)
Catalog# ET1611-61
Rabbit Monoclonal to CD13
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WB
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IHC-P
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IP
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Human
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Mouse
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Rat
Western blot analysis of CD13 on different lysates with Rabbit anti-CD13 antibody (ET1611-61) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-CD13 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 110 kDa
Observed band size: 150 kDa
Exposure time: 110 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-61) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
CD13 Recombinant Rabbit Monoclonal Antibody [SN71-04] (ET1611-61)
Immunogen
Synthetic peptide within human cd13 aa 401-450 / 967.
Host
Rabbit
Positive Control
PANC-1 cell lysate, mouse kidney tissue lysate, rat kidney tissue lysate, human tonsil tissue, human liver tissue, human breast tissue, human kidney tissue, mouse pancreas tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SN71-04
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:5,000
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IHC-P
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1:100-1:500
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IP
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1-2μg/sample
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 110 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
CD13
SYNONYMS
Alanyl (membrane) aminopeptidase antibody;Alanyl aminopeptidase antibody;Aminopeptidase M antibody;Aminopeptidase N antibody;AMPN_HUMAN antibody;ANPEP antibody;AP M antibody;AP N antibody;AP-M antibody;AP-N antibody;APN antibody;CD 13 antibody;CD13 antibody;CD13 antigen antibody;gp150 antibody;hAPN antibody;LAP 1 antibody;LAP1 antibody;Microsomal aminopeptidase antibody;Myeloid plasma membrane glycoprotein CD13 antibody;p150 antibody;PEPN antibody
SEQUENCE SIMILARITIES
Belongs to the peptidase M1 family.
TISSUE SPECIFICITY
Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.
POST-TRANSLATIONAL MODIFICATION
Sulfated.; N- and O-glycosylated.; May undergo proteolysis and give rise to a soluble form.
SUBCELLULAR LOCATION
Cell membrane.
FUNCTION
CD13, or aminopeptidase N, is a type II transmembrane glycoprotein that is expressed on most cells of Myeloid origin, including monocytes, basophils, eosinophils, neutrophils and Myeloid leukemias. CD13 is also found on certain epithelial cells, fibroblasts and osteoclasts. CD13 acts as a zinc-binding metalloprotease that plays a role in digestion and may function in the inactivation of some regulatory peptides such as enkephalins. CD13 may play a role in the invasion of cancer cells by enhancing their invasive capacity and metastatic behavior. The activity of CD13 can be inactivated using specific inhibitors that evoke apoptosis of CD13-positive cancer cells. Basic fibroblast growth factor (bFGF) expression upregulates CD13 expression in human melanoma cells by activating both the Myeloid and the epithelial CD13 promoter.
CITATIONS
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Zheng Youle,et al
Anti-Coronavirus Potential of Polyether Ionophores: The New Application of Veterinary Antibiotics in Livestock
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WB
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Pig
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Citation
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