CD11b Mouse Monoclonal Antibody [A2-B5-D2] (EM1902-35)
Catalog# EM1902-35
Mouse Monoclonal to CD11b
Tested for
-
WB
Reacts with
-
Human
-
Mouse
Western blot analysis of CD11b on different lysates with Mouse anti-CD11b antibody (EM1902-35) at 1/500 dilution.
Lane 1: RAW264.7 cell lysate (RIPA lysis)
Lane 2: RAW264.7 cell lysate (hot lysis)
Lane 3: RAW264.7 cell lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 127 kDa
Observed band size: 170 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-35) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature.
Lane 1: RAW264.7 cell lysate (RIPA lysis)
Lane 2: RAW264.7 cell lysate (hot lysis)
Lane 3: RAW264.7 cell lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 127 kDa
Observed band size: 170 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-35) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature.