PRODUCT CODE: EM1902-35

CD11b Mouse Monoclonal Antibody [A2-B5-D2] (EM1902-35)

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

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Western blot analysis of CD11b on Mouse spleen tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-35, 1/100) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of CD11b on Mouse spleen tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-35, 1/100) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Flow cytometric analysis of CD11b was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1902-35, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa FluorTM488 Goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; blcak).
Western blot analysis of CD11b on Mouse spleen tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-35, 1/100) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

CD11b Mouse Monoclonal Antibody [A2-B5-D2] (EM1902-35)

Immunogen

Recombinant protein within mouse cd11b aa 1-300.

Host

Mouse

Positive Control

Mouse spleen tissue lysates, A549.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A2-B5-D2

PROPERTIES

Form

Liquid

Storage Condition

Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A/G purified.

MOLECULAR WEIGHT

Predicted band size: 125 kDa.

Isotype

IgM

APPLICATION DILUTION

  • WB

  • 1:500

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

CD11b

SYNONYMS

antigen CD11b (p170) antibody; Antigen CD11b p170 antibody; CD11 antigen like family member B antibody; CD11 antigen-like family member B antibody; CD11b antibody; CD11b/CD18 antibody; CD49d antibody; Cell surface glycoprotein MAC-1 subunit alpha antibody; Complement component 3 receptor 3 subunit antibody; Complement Component Receptor 3 Alpha antibody; Complement receptor type 3 antibody; Complement receptor type 3, alpha subunit antibody; CR 3 alpha chain (CR3A) antibody; CR 3 alpha chain antibody; CR-3 alpha chain antibody; CR3 antibody; CR3A antibody; F730045J24Rik antibody; Integrin Alpha M antibody; Integrin alpha M chain antibody; Integrin alpha-M antibody; Integrin beta 2 alpha subunit antibody; Integrin subunit alpha M antibody; integrin, alpha M (complement component 3 receptor 3 subunit) antibody; ITAM_HUMAN antibody; ITGAM antibody; Leukocyte adhesion receptor MO1 antibody; Ly-40 antibody; MAC 1 antibody; Mac-1a antibody; MAC1 antibody; Mac1, alpha subunit antibody; MAC1A antibody; Macrophage antigen alpha polypeptide antibody; MGC117044 antibody; Mo1 antibody; Mo1, alpha subunit antibody; MO1A antibody; Neutrophil adherence receptor alpha M subunit antibody; Neutrophil adherence receptor antibody; SLEB6 antibody

SUBCELLULAR LOCATION

Cell membrane, Membrane.

FUNCTION

Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (By similarity). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration. In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (By similarity). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development .