CD11b Mouse Monoclonal Antibody [A2-B5-D2] (EM1902-35)
Catalog# EM1902-35
Mouse Monoclonal to CD11b
Tested for
-
WB
-
FC
Reacts with
-
Human
-
Mouse
Western blot analysis of CD11b on RAW264.7 cell lysates with Mouse anti-CD11b antibody (EM1902-35) at 1/500 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 125 kDa
Observed band size: 150 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-35) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 125 kDa
Observed band size: 150 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-35) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
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