CD11b Mouse Monoclonal Antibody [4-6] (EM1701-42)
Catalog# EM1701-42
Mouse Monoclonal to CD11b
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IHC-P
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IF-Cell
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FC
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WB
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IF-Tissue
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Human
Lane 1: TF-1 cell lysate
Lane 2: THP-1 cell lysate
Lane 3: U-937 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 127 kDa
Observed band size: 170 kDa
Exposure time: 30 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-42) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
CD11b Mouse Monoclonal Antibody [4-6] (EM1701-42)
Immunogen
Recombinant protein within human cd11b aa 544-743 / 1,152.
Host
Mouse
Positive Control
TF-1 cell lysate, THP-1 cell lysate, U-937 cell lysate, A431, LOVO, human tonsil tissue, human spleen tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
4-6
RRID
APPLICATION DILUTION
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
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FC:1:50-1:100
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WB
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1:1,000
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IF-Tissue
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1:50
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein G affinity purified.
Molecular Weight
Predicted band size: 127 kDa
Isotype
IgG2b
TARGET
UNIPROT #
PROTEIN NAME
CD11b
SYNONYMS
antigen CD11b (p170) antibody;Antigen CD11b p170 antibody;CD11 antigen like family member B antibody;CD11 antigen-like family member B antibody;CD11b antibody;CD11b/CD18 antibody;CD49d antibody;Cell surface glycoprotein MAC-1 subunit alpha antibody;Complement component 3 receptor 3 subunit antibody;Complement Component Receptor 3 Alpha antibody;Complement receptor type 3, alpha subunit antibody;CR 3 alpha chain (CR3A) antibody;CR 3 alpha chain antibody;CR-3 alpha chain antibody;CR3 antibody;CR3A antibody;F730045J24Rik antibody;Integrin Alpha M antibody;Integrin alpha M chain antibody;Integrin alpha-M antibody;Integrin beta 2 alpha subunit antibody;Integrin subunit alpha M antibody;integrin, alpha M (complement component 3 receptor 3 subunit) antibody;ITAM_HUMAN antibody;ITGAM antibody;Leukocyte adhesion receptor MO1 antibody;Ly-40 antibody;MAC 1 antibody;Mac-1a antibody;MAC1 antibody;Mac1, alpha subunit antibody;MAC1A antibody;Macrophage antigen alpha polypeptide antibody;MGC117044 antibody;Mo1, alpha subunit antibody;MO1A antibody;Neutrophil adherence receptor alpha M subunit antibody;Neutrophil adherence receptor antibody;SLEB6 antibody
SEQUENCE SIMILARITIES
Belongs to the integrin alpha chain family.
TISSUE SPECIFICITY
Predominantly expressed in monocytes and granulocytes. Expressed in neutrophils (at protein level).
SUBCELLULAR LOCATION
Cell membrane.
FUNCTION
Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration. In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils. May regulate phagocytosis-induced apoptosis in extravasated neutrophils. May play a role in mast cell development.
CITATIONS
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Optimized CAR-T therapy based on spatiotemporal changes and chemotactic mechanisms of MDSCs induced by hypofractionated radiotherapy
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FC
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Mouse
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Citation
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