PRODUCT CODE: ET1610-1

CD11a Recombinant Rabbit Monoclonal Antibody [SC56-01] (ET1610-1)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

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Western blot analysis of CD11a on Jurkat cell lysates using anti-CD11a antibody at 1/1,000 dilution.
  • Western blot analysis of CD11a on Jurkat cell lysates using anti-CD11a antibody at 1/1,000 dilution.
  • ICC staining CD11a in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD11a in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD11a in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD11a antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD11a antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of K562 cells with CD11a antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of CD11a on Jurkat cell lysates using anti-CD11a antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

CD11a Recombinant Rabbit Monoclonal Antibody [SC56-01] (ET1610-1)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Jurkat, SW480, Hela, HepG2, human spleen tissue, human tonsil tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC56-01

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

180 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

CD11a

SYNONYMS

Lymphocyte function associated antigen, type 1, alpha subunit antibody; Antigen CD11A (p180), lymphocyte function associated antigen 1, alpha polypeptide antibody; Antigen CD11A antibody; CD 11a antibody; CD11 antigen-like family member A antibody; CD11a antibody; CD11a antigen antibody; Integrin Alpha L antibody; Integrin alpha-L antibody; Integrin gene promoter antibody; Integrin, alpha L (antigen CD11A (p180), lymphocyte function associated antigen 1; alpha polypeptide) antibody; ITAL_HUMAN antibody; Itgal antibody; ITGAL protein antibody; Leukocyte adhesion glycoprotein LFA 1 alpha chain antibody; Leukocyte adhesion glycoprotein LFA-1 alpha chain antibody; Leukocyte Adhesion Glycoprotein LFA1 Alpha Chain antibody; Leukocyte function associated molecule 1 alpha chain antibody; Leukocyte function-associated molecule 1 alpha chain antibody; LFA 1 alpha (LFA1A) antibody; LFA 1 alpha antibody; LFA 1 antibody; LFA 1A antibody; LFA-1A antibody; LFA1A antibody; Ly15 antibody; Ly21 antibody; Lymphocyte function associated antigen 1 antibody; Lymphocyte Function Associated Antigen Type 1 alpha antibody; lymphocyte function-associated antigen 1, alpha polypeptide antibody; p180 antibody

SEQUENCE SIMILARITIES

Belongs to the integrin alpha chain family.

TISSUE SPECIFICITY

Leukocytes.

POST-TRANSLATIONAL MODIFICATION

In resting T-cells, up to 40% of surface ITGAL is constitutively phosphorylated. Phosphorylation causes conformational changes needed for ligand binding and is necessary for activation by some physiological agents.

SUBCELLULAR LOCATION

Membrane.

FUNCTION

Integrins are heterodimers composed of noncovalently associated transmembrane a and b subunits. The 16 a and 8 b subunits heterodimerize to produce more than 20 different receptors. Most integrin receptors bind ligands that are components of the extracellular matrix, including Fibronectin, Collagen and Vitronectin. Certain integrins can also bind to soluble ligands such as Fibrinogen, or to counterreceptors on adjacent cells such as the intracellular adhesion molecules (ICAMs), leading to aggregation of cells. Ligands serve to cross-link or cluster integrins by binding to adjacent integrin receptors; both receptor clustering and ligand occupancy are necessary for the activation of integrin-mediated responses. In addition to mediating cell adhesion and cytoskeletal organization, integrins function as signaling receptors. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis.