PRODUCT CODE: HA720072

CD105 Recombinant Rabbit Monoclonal Antibody [JE60-59] (HA720072)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of CD105 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFTM/TBST for 1 hour at room temperature. The primary antibody (HA720072, 1/500) was used in 5% NFTM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Human placenta tissue lysate<br />
Lane 2: JAR cell lysate
  • Western blot analysis of CD105 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFTM/TBST for 1 hour at room temperature. The primary antibody (HA720072, 1/500) was used in 5% NFTM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Human placenta tissue lysate<br />
Lane 2: JAR cell lysate
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD105 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720072, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-CD105 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720072, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of CD105 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFTM/TBST for 1 hour at room temperature. The primary antibody (HA720072, 1/500) was used in 5% NFTM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Human placenta tissue lysate
Lane 2: JAR cell lysate

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

CD105 Recombinant Rabbit Monoclonal Antibody [JE60-59] (HA720072)

Immunogen

Recombinant protein.

Host

Rabbit

Positive Control

Human placenta tissue lysate, JAR cell lysate, human kidney tissue, human placenta tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JE60-59

PROPERTIES

Form

Liquid

Storage Condition

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

71 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

CD105

SYNONYMS

AI528660 antibody;AI662476 antibody;CD 105 antibody;CD105 antibody;CD105 antigen antibody;EGLN_HUMAN antibody;END antibody;Endoglin antibody;Eng antibody;FLJ41744 antibody;HHT1 antibody;ORW antibody;ORW1 antibody;Osler Rendu Weber syndrome 1 antibody;RP11 228B15.2 antibody;S endoglin antibody;S-endoglin antibody;SN6 antibody

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds to the beta1 and beta3 peptides with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. This gene may also be involved in preeclampsia and several types of cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.