CD100 Recombinant Rabbit Monoclonal Antibody [JE63-21] (HA721013)
Catalog# HA721013
Rabbit Monoclonal to CD100
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WB
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Human
Lane 1: Jurkat cell lysate
Lane 2: Daudi cell lysate
Lane 3: HL-60 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 96 kDa
Observed band size: 130 kDa
Exposure time: 30 seconds;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721013) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
CD100 Recombinant Rabbit Monoclonal Antibody [JE63-21] (HA721013)
Immunogen
Synthetic peptide within human cd100 aa 126-175/862.
Host
Rabbit
Positive Control
Jurkat cell lysate, Daudi cell lysate, HL-60 cell lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE63-21
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 96 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
CD100
SYNONYMS
A8 antibody;BB 18 antibody;BB18 antibody;CD 100 antibody;CD100 antibody;CD100 antigen antibody;Coll 4 antibody;COLL4 antibody;Collapsin 4 antibody;Collapsin4 antibody;GR3 antibody;Leukocyte activation antigen CD100 antibody;M sema G antibody;MSEMA antibody;SEM4D_HUMAN antibody;Sema 4d antibody;Sema domain immunoglobulin domain Ig transmembrane domain TM and short cytoplasmic domain semaphorin 4D antibody;Sema H antibody;SEMA J antibody;Sema4d antibody;Semacl 2 antibody;Semacl2 antibody;SemaH antibody;SEMAJ antibody;Semaphorin 4D antibody;Semaphorin C like 2 antibody;Semaphorin H antibody;Semaphorin J antibody;Semaphorin-4D antibody;Semaphorin4D antibody;SemaphorinJ antibody;Semcl 2 antibody;Semcl2 antibody
SUBCELLULAR LOCATION
Cell membrane.
FUNCTION
Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling. Regulates GABAergic synapse development (By similarity). Promotes the development of inhibitory synapses in a PLXNB1-dependent manner (By similarity). Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA. Promotes the migration of cerebellar granule cells. Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway.
CITATIONS
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Huang Jiaxing,et al
YY1 Lactylation Aggravates Autoimmune Uveitis by Enhancing Microglial Functions via Inflammatory Genes
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WB
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Human
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Mouse
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Citation
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