CD10 Recombinant Mouse Monoclonal Antibody - BSA and Azide free [A1G3-R] (HA610121)
Catalog# HA610121
Mouse Monoclonal to CD10
Tested for
-
WB
-
IF-Cell
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of CD10 on different lysates with Mouse anti-CD10 antibody (HA610121) at 1/2,000 dilution.
Lane 1: Daudi cell lysate (20 µg/Lane)
Lane 2: Ramos cell lysate (20 µg/Lane)
Lane 3: LNCaP cell lysate (20 µg/Lane)
Lane 4: HT-29 cell lysate (negative) (20 µg/Lane)
Lane 5: Mouse kidney tissue lysate (40 µg/Lane)
Predicted band size: 86 kDa
Observed band size: 100 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610121) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of CD10 on different lysates with Mouse anti-CD10 antibody (HA610121) at 1/2,000 dilution.
Lane 1: Daudi cell lysate (20 µg/Lane)
Lane 2: Ramos cell lysate (20 µg/Lane)
Lane 3: LNCaP cell lysate (20 µg/Lane)
Lane 4: HT-29 cell lysate (negative) (20 µg/Lane)
Lane 5: Mouse kidney tissue lysate (40 µg/Lane)
Predicted band size: 86 kDa
Observed band size: 100 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610121) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.