PRODUCT CODE: ET1602-22

CCR7 Recombinant Rabbit Monoclonal Antibody [SR36-04] (ET1602-22)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of CCR7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-22, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Jurkat cell lysate<br />
Lane 2: MCF-7 cell lysate<br />
Lane 3: PC-12 cell lysate<br />
Lane 4: Daudi cell lysate
  • Western blot analysis of CCR7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-22, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Jurkat cell lysate<br />
Lane 2: MCF-7 cell lysate<br />
Lane 3: PC-12 cell lysate<br />
Lane 4: Daudi cell lysate
  • ICC staining of CCR7 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-22, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of CCR7 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-22, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CCR7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-22, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CCR7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-22, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of CCR7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-22, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Jurkat cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: PC-12 cell lysate
Lane 4: Daudi cell lysate

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

CCR7 Recombinant Rabbit Monoclonal Antibody [SR36-04] (ET1602-22)

Immunogen

Synthetic peptide within human ccr7 aa 25-55.

Host

Rabbit

Positive Control

Jurkat cell lysate, MCF-7 cell lysate, PC-12 cell lysate, Daudi cell lysate, Hela, MCF-7, human tonsil tissue, human spleen tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SR36-04

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

43 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • IP

  • assay-dependent

TARGET

UNIPROT #

PROTEIN NAME

CCR7

SYNONYMS

BLR 2 antibody; BLR2 antibody; C C chemokine receptor type 7 antibody; C C CKR 7 antibody; C-C chemokine receptor type 7 antibody; C-C CKR-7 antibody; CC chemokine receptor 7 antibody; CC chemokine receptor type 7 antibody; CC CKR 7 antibody; CC-CKR-7 antibody; CCCKR7 antibody; CCR 7 antibody; CCR-7 antibody; Ccr7 antibody; CCR7_HUMAN antibody; CD 197 antibody; CD197 antibody; CD197 antigen antibody; CDw197 antibody; Chemokine (C C motif) receptor 7 antibody; Chemokine (C C) receptor 7 antibody; Chemokine C C motif receptor 7 antibody; Chemokine C C receptor 7 antibody; Chemokine receptor 7 antibody; Chemokine receptor 7-like protein antibody; CMKBR7 antibody; EBI 1 antibody; EBI1 antibody; Ebi1h antibody; EBV Induced G Protein Coupled Receptor 1 antibody; EBV-induced G-protein coupled receptor 1 antibody; Epstein Barr virus induced G protein coupled receptor antibody; Epstein Barr virus induced gene 1 antibody; Epstein-Barr virus-induced G-protein coupled receptor 1 antibody; EVI 1 antibody; EVI1 antibody; Lymphocyte Specific G Protein Coupled Peptide Receptor antibody; MGC108519 antibody; MIP 3 beta receptor antibody; MIP-3 beta receptor antibody; MIP3 Beta Receptor antibody

SEQUENCE SIMILARITIES

Belongs to the G-protein coupled receptor 1 family.

TISSUE SPECIFICITY

Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.

SUBCELLULAR LOCATION

Cell membrane

FUNCTION

C-C or ∫ chemokine family members are characterized by a pair of adjacent cysteine residues and serve as potent chemoattractants and activators of monocytes and T cells. C-C chemokine receptor family members include CKR-1, CKR-2A, CKR-2B, CKR-3, CKR-4, CKR-5, CKR-6, CKR-7, CKR-8, CKR-9, CKR-10 and the Duffy blood group antigen. Each of these receptors are G protein-coupled, seven pass transmembrane domain proteins whose major physiological role is to function in the chemotaxis of T cells and phagocytic cells to areas of inflammation. However, this receptor family has also been shown to facilitate viral infection. CKR-7 (C-C chemokine receptor type 7), also known as CCR7, CMKBR7, EBI1 or EVI1, is a 378 amino acid multi-pass membrane protein that belongs to the CC-chemokine receptor family. Expressed in activated B- and T-lymphocytes, as well as in various lymphoid tissues, CKR-7 functions as a receptor for MIP-3∫ and is thought to be involved in mediating normal lymphocyte function.