CCL2 / MCP-1 Rabbit Polyclonal Antibody (HA500267)
Catalog# HA500267
Rabbit Polyclonal to CCL2
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WB
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IF-Cell
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FC
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Mouse
Lane 1: RAW264.7 cell lysate
Lane 2: RAW264.7 treated with 100nM TPA overnight then 100ng/mL LPS for 7 hours add 1μg/mL BFA for last 3 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 16 kDa
Observed band size: 16 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500267) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
CCL2 / MCP-1 Rabbit Polyclonal Antibody (HA500267)
Immunogen
Recombinant protein within rat ccl2 aa 1-148.
Host
Rabbit
Positive Control
RAW264.7 treated with 100nM TPA overnight then 100ng/mL LPS for 7 hours add 1μg/mL BFA for last 3 hours cell lysate, RAW264.7 cells treated with 100nM TPA overnight then 100ng/mL LPS for 7 hours add 1μg/mL BFA for last 3 hours.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:5,000
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IF-Cell
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1:500
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 16 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
CCL2
SYNONYMS
C-C motif chemokine 2 antibody;CCL2 antibody;CCL2_HUMAN antibody;Chemokine (C C motif) ligand 2 antibody;GDCF 2 antibody;GDCF-2 antibody;GDCF2 antibody;HC11 antibody;HSMCR30 antibody;JE antibody;MCAF antibody;MCP 1 antibody;MCP-1 antibody;MCP1 antibody;MGC9434 antibody;Monocyte chemoattractant protein 1 antibody;Monocyte chemotactic and activating factor antibody;Monocyte chemotactic protein 1 antibody;Monocyte secretory protein JE antibody;SCYA2 antibody;Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je) antibody;Small inducible cytokine A2 antibody;Small inducible cytokine subfamily A (Cys Cys), member 2 antibody;Small-inducible cytokine A2 antibody;SMC CF antibody;SMC-CF antibody;SMCCF antibody
SUBCELLULAR LOCATION
Secreted.
FUNCTION
CCL2 exhibits a chemotactic activity for monocytes and basophils. However, it does not attract neutrophils or eosinophils. After deletion of the N-terminal residue, CCL2 loses its attractivity for basophils and becomes a chemoattractant of eosinophils. Basophils and mast cells that are treated with CCL2 release their granules to the intercellular space. This effect can be also potentiated by a pre-treatment with IL-3 or even by other cytokines. CCL2 augments monocyte anti-tumor activity and it is essential for formation of granulomas. CCL2 protein become a CCR2 antagonist when it is cleaved by metalloproteinase MMP-12.
CITATIONS
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Wu, Q., Xu, R., Zhang, K., Sun, R., Yang, M., Li, K., Liu, H., Xue, Y., Xu, H., & Guo, Y.
Characterization of early myocardial inflammation in ischemia-reperfusion injury
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WB
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Mouse
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Citation
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Linxi Lv , Hui Jiang , Dianze Song ,et al
Sirt3 improves monosodium urate crystal-induced inflammation by suppressing Acod1 expression
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WB
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Mouse
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Citation
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