PRODUCT CODE: ET7111-22

CC2D1A Recombinant Rabbit Monoclonal Antibody [JE55-34] (ET7111-22)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

Western blot analysis of CC2D1A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7111-22, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: MCF-7 cell lysate<br />
Lane 2: A431 cell lysate<br />
Lane 3: PC-12 cell lysate
  • Western blot analysis of CC2D1A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7111-22, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: MCF-7 cell lysate<br />
Lane 2: A431 cell lysate<br />
Lane 3: PC-12 cell lysate
  • Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-CC2D1A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-22, 1/200)  for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CC2D1A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-22, 1/50)  for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of CC2D1A was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7111-22, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of CC2D1A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7111-22, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: MCF-7 cell lysate
Lane 2: A431 cell lysate
Lane 3: PC-12 cell lysate

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

CC2D1A Recombinant Rabbit Monoclonal Antibody [JE55-34] (ET7111-22)

Immunogen

Recombinant protein within c-terminal human cc2d1a .

Host

Rabbit

Positive Control

MCF-7 cell lysate, A431 cell lysate, PC-12 cell lysate, human appendix tissue, human tonsil tissue, MCF-7.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JE55-34

PROPERTIES

Form

Liquid

Storage Condition

Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

Predicted band size: 104 kDa.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

CC2D1A

SYNONYMS

Akt kinase interacting protein 1 antibody; Coiled coil and C2 domain containing 1A antibody; Coiled coil and C2 domain containing protein 1A antibody; Five repressor element under dual repression binding protein 1 antibody; FLJ20241 antibody; FLJ41160 antibody; FRE under dual repression binding protein 1 antibody; FREUD 1 antibody; Freud 1/Aki1 antibody; Mental retardation, nonsyndromic, autosomal recessive, 3 antibody; MRT3 antibody; Putative NF kappa B activating protein 023N antibody; Putative NF kappa B activating protein antibody

SUBCELLULAR LOCATION

Centrosome, Nucleus, Cytoplasm.

FUNCTION

This gene encodes a transcriptional repressor that binds to a conserved 14-bp 5'-repressor element and regulates expression of the 5-hydroxytryptamine (serotonin) receptor 1A gene in neuronal cells. The DNA binding and transcriptional repressor activities of the protein are inhibited by calcium. A mutation in this gene results in a nonsyndromic form of cognitive disability (MRT3).