PRODUCT CODE: ET7108-87

CBFb Recombinant Rabbit Monoclonal Antibody [JG39-35] (ET7108-87)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

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Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-CBFb antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-CBFb antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CBFb antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SiHa cells with CBFb antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).  Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-CBFb antibody. Counter stained with hematoxylin.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

CBFb Recombinant Rabbit Monoclonal Antibody [JG39-35] (ET7108-87)

Immunogen

Recombinant protein within human cbfb aa 1-200.

Host

Rabbit

Positive Control

Rat kidney tissue, human kidney tissue, SiHa.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JG39-35

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

22 kDa

Isotype

IgG

APPLICATION DILUTION

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • WB

  • 1:500

TARGET

UNIPROT #

PROTEIN NAME

CBFb

SYNONYMS

CBF b antibody; CBF beta antibody; CBF-beta antibody; CBFB antibody; CBFbeta antibody; Core binding factor beta subunit antibody; core binding factor subunit beta antibody; Core-binding factor subunit beta antibody; PEA 2 antibody; PEA2 antibody; PEA2 beta antibody; PEA2-beta antibody; PEA2beta antibody; PEBB_HUMAN antibody; PEBP 2B antibody; PEBP2 beta antibody; PEBP2-beta antibody; PEBP2B antibody; PEBP2beta antibody; Polyomavirus enhancer binding protein 2 beta subunit antibody; Polyomavirus enhancer-binding protein 2 beta subunit antibody; SL3 3 enhancer factor 1 beta subunit antibody; SL3 3 enhancer factor 1 subunit beta antibody; SL3-3 enhancer factor 1 subunit beta antibody; SL3/AKV core binding factor beta subunit antibody; SL3/AKV core-binding factor beta subunit antibody

SEQUENCE SIMILARITIES

Belongs to the CBF-beta family.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

The transcription factor Polyomavirus enhancer binding protein 2 (PEBP2), also designated Osf2 (Osteoblast-specific transcription factor), CBFA1 (Core Binding Factor) and AML3 (Acute myeloid leukemia), is composed of two subunits, a and b, which are essential for the regulation of hematopoiesis and osteogenesis. The PEBP2a subunits, PEBP2aA, PEBP2aB and PEBP2aC, are encoded by three RUNX genes, all of which contain a 128-amino acid region homologous to the highly conserved Drosophila segmentation gene, Runt. This region is involved in DNA binding and heterodimerization with the regulatory b subunit, which facilitates DNA binding of the a subunit. Both subunits are required for in vivo function; the disruption of either gene results in a lack of definitive hematopoiesis followed by embryo death in utero due to hemorrhage in the central nervous system. The gene encoding PEBP2b is the target of chromosomal inversion 16 (p13;q22) with the smooth muscle myosin heavy chain, producing a chimeric gene, PEBP2b/CBFB-SMMHC, that is associated with human acute myeloid leukemia.