PRODUCT CODE: ET1607-15

Caveolin-2 Recombinant Rabbit Monoclonal Antibody [SY22-05] (ET1607-15)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

Western blot analysis of caveolin-2 on HUVEC cell lysates using anti-caveolin-2 antibody at 1/1,000 dilution.
  • Western blot analysis of caveolin-2 on HUVEC cell lysates using anti-caveolin-2 antibody at 1/1,000 dilution.
  • ICC staining caveolin-2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human cervix uteri tissue using anti-caveolin-2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-caveolin-2 antibody. Counter stained with hematoxylin.
Western blot analysis of caveolin-2 on HUVEC cell lysates using anti-caveolin-2 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Caveolin-2 Recombinant Rabbit Monoclonal Antibody [SY22-05] (ET1607-15)

Immunogen

Synthetic peptide within human caveolin-2 aa 1-50 / 162.

Host

Rabbit

Positive Control

HUVEC, Hela, human cervix uteri tissue, human uterus tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SY22-05

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

20 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Caveolin-2

SYNONYMS

CAV antibody; CAV2 antibody; CAV2_HUMAN antibody; Caveolae protein 20 kD antibody; Caveolin 2 antibody; Caveolin 2 isoform a and b antibody; Caveolin-2 antibody; MGC12294 antibody; OTTHUMP00000025032 antibody; OTTHUMP00000195982 antibody

SEQUENCE SIMILARITIES

Belongs to the caveolin family.

TISSUE SPECIFICITY

Expressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on serine and tyrosine residues. CAV1 promotes phosphorylation on Ser-23 which then targets the complex to the plasma membrane, lipid rafts and caveolae. Phosphorylation on Ser-36 appears to modulate mitosis in endothelial cells (By similarity). Phosphorylation on both Tyr-19 and Tyr-27 is required for insulin-induced 'Ser-727' phosphorylation of STAT3 and its activation. Phosphorylation on Tyr-19 is required for insulin-induced phosphorylation of MAPK1 and DNA binding of STAT3. Tyrosine phosphorylation is induced by both EGF and insulin (By similarity).

SUBCELLULAR LOCATION

Cytoplasm, Nucleus, Membrane.

FUNCTION

Caveolae (also known as plasmalemmal vesicles) are 50-100 nM flask-shaped membranes that represent a subcompartment of the plasma membrane. On the basis of morphological studies, caveolae have been implicated to function in the transcytosis of various macromolecules (including LDL) across capillary endothelial cells, uptake of small molecules via potocytosis and the compartmentalization of certain signaling molecules including G protein-coupled receptors. Three proteins, caveolin-1, caveolin-2 and caveolin-3, have been identified as principal components of caveolae. Two forms of caveolin-1, designated a and b, share a distinct but overlapping cellular distribution and differ by an N-terminal 31 amino acid sequence that is absent from the b isoform. Caveolin-1 shares 31% identity with caveolin-2 and 65% identity with caveolin-3 at the amino acid level. Functionally, the three proteins differ in their interactions with heterotrimeric G protein isoforms.