Caveolin-1 Recombinant Rabbit Monoclonal Antibody [SZ02-01] (ET1603-1)
Catalog# ET1603-1
Rabbit Monoclonal to Caveolin-1
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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Human
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Mouse
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Rat
Lane 1/2: A549 cell lysate
Lane 3/4: A431 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 20 kDa
Observed band size: 20 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-1) at different dilutions were used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Caveolin-1 Recombinant Rabbit Monoclonal Antibody [SZ02-01] (ET1603-1)
Immunogen
Synthetic peptide within human caveolin-1 aa 129-178 / 178.
Host
Rabbit
Positive Control
A549 cell lysate, A431 cell lysate, human lung tissue lysate, mouse lung tissue lysate, mouse heart tissue lysate, rat lung tissue lysate, rat heart tissue lysate, A549, MCF-7, NIH/3T3, human liver tissue, human liver carcinoma tissue, human uterus tissue, mouse heart tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SZ02-01
RRID
APPLICATION DILUTION
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WB
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1:500-1:5,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:1,000
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IHC-P
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1:200-1:10,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 20 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Caveolin-1
SYNONYMS
BSCL3 antibody;CAV antibody;CAV1 antibody;CAV1_HUMAN antibody;caveolae protein, 22 kD antibody;caveolin 1 alpha isoform antibody;caveolin 1 beta isoform antibody;Caveolin 1 caveolae protein 22kDa antibody;Caveolin-1 antibody;Caveolin1 antibody;cell growth-inhibiting protein 32 antibody;CGL3 antibody;LCCNS antibody;MSTP085 antibody;OTTHUMP00000025031 antibody;PPH3 antibody;VIP 21 antibody;VIP21 antibody
SEQUENCE SIMILARITIES
Belongs to the caveolin family.
TISSUE SPECIFICITY
Skeletal muscle, liver, stomach, lung, kidney and heart (at protein level). Expressed in the brain.
POST-TRANSLATIONAL MODIFICATION
Ubiquitinated. Undergo monoubiquitination and multi- and/or polyubiquitination. Monoubiquitination of N-terminal lysines promotes integration in a ternary complex with UBXN6 and VCP which promotes oligomeric CAV1 targeting to lysosomes for degradation.; The initiator methionine for isoform 2 is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.; Phosphorylated at Tyr-14 by ABL1 in response to oxidative stress.
SUBCELLULAR LOCATION
Golgi apparatus membrane, Cell membrane, Membrane.
FUNCTION
Caveolae (also known as plasmalemmal vesicles) are 50-100 nM flask-shaped membranes that represent a subcompartment of the plasma membrane. On the basis of morphological studies, caveolae have been implicated to function in the transcytosis of various macromolecules (including LDL) across capillary endothelial cells, uptake of small molecules via potocytosis and the compartmentalization of certain signaling molecules including G protein-coupled receptors. Three proteins, caveolin-1, caveolin-2 and caveolin-3, have been identified as principal components of caveolae. Two forms of caveolin-1, designated alpha and beta, share a distinct but overlapping cellular distribution and differ by an amino terminal 31 amino acid sequence which is absent from the beta isoform. Caveolin-1 shares 31% identity with caveolin-2 and 65% identity with caveolin-3 at the amino acid level. Functionally, the three proteins differ in their interactions with heterotrimeric G protein isoforms.
CITATIONS
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Plasma TNFRSF11B as a New Predictive Inflammatory Marker of Sepsis-ARDS with Endothelial Dysfunction
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WB
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Human
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Mouse
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Citation
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