Cathepsin S Rabbit Polyclonal Antibody (HA500422)
Catalog# HA500422
Rabbit Polyclonal to Cathepsin S
-
WB
-
IF-Cell
-
Human
-
Mouse
-
Rat
Lane 1: THP-1 cell lysate (10 µg/Lane)
Lane 2: THP-1 cell lysate (10 µg/Lane)
Lane 3: Hela cell lysate (10 µg/Lane)
Lane 4: A431 cell lysate (10 µg/Lane)
Lane 5: PC-12 cell lysate (10 µg/Lane)
Lane 6: Rat brain tissue lysate (20 µg/Lane)
Lane 7: Mouse brain tissue lysate (20 µg/Lane)
Lysates/proteins at 10 µg/Lane.
Predicted band size: 37 kDa
Observed band size: 25/35/40 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500422) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
Cathepsin S Rabbit Polyclonal Antibody (HA500422)
Immunogen
Recombinant protein within human cathepsin s aa 101-331 / 331.
Host
Rabbit
Positive Control
THP-1 cell lysate, Hela cell lysate, A431 cell lysate, PC-12 cell lysate, rat brain tissue lysate, mouse brain tissue lysate, SiHa.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
-
WB
-
1:1,000
-
IF-Cell
-
1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2.305ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 37 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Cathepsin S
SYNONYMS
Cathepsin S antibody;CathepsinS antibody;CATS_HUMAN antibody;Ctss antibody
SUBCELLULAR LOCATION
Lysosome, Secreted, Cytoplasmic vesicle, phagosome.
FUNCTION
Cathepsin S is a protein that in humans is encoded by the CTSS gene. Transcript variants utilizing alternative polyadenylation signals exist for this gene. Cathepsin S is a member of the peptidase C1 family of cysteine cathepsins, a lysosomal cysteine protease that may participate in the degradation of antigenic proteins to peptides for presentation to the MHC class II. Cathepsin S can function as an elastase over a broad pH range in alveolar macrophages. Cathepsin S is a lysosomal enzyme that belongs to the papain-like protease of cysteine proteases. While a role in antigen presentation has long been recognized, it is now understood that cathepsin S has a role in itch and pain, or nociception . The nociceptive activity results from cathepsin S functioning as a signaling molecule via activation of protease-activated receptors 2 and 4 members of the G-protein coupled receptor family.
CITATIONS
-
Weng Chunyan,et al
The PDIA3-STAT3 protein complex regulates IBS formation and development via CTSS/MHC-II pathway-mediated intestinal inflammation
-
WB
-
Mouse
-
Citation
-
