PRODUCT CODE: R1407-1

Cathepsin B Rabbit Polyclonal Antibody (R1407-1)

Applications

  • WB

  • ICC

REACTIVITY

  • Human

Western blot analysis on cell lysates using anti- Cathepsin B rabbit polyclonal antibodies.
  • Western blot analysis on cell lysates using anti- Cathepsin B rabbit polyclonal antibodies.
  • ICC staining Cathepsin B in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
Western blot analysis on cell lysates using anti- Cathepsin B rabbit polyclonal antibodies.

Applications

  • WB

  • ICC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Cathepsin B Rabbit Polyclonal Antibody (R1407-1)

Immunogen

Synthetic peptide (klh-coupled) within human cathepsin b c-terminal.

Host

Rabbit

Positive Control

Hela, SW480, A549

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

38 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1000

  • ICC

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

Cathepsin B

SYNONYMS

Amyloid precursor protein secretase antibody; APP secretase antibody; APPS antibody; CATB_HUMAN antibody; Cathepsin B heavy chain antibody; Cathepsin B1 antibody; CathepsinB antibody; CPSB antibody; CTSB antibody; cysteine protease antibody; OTTHUMP00000116009 antibody; OTTHUMP00000229510 antibody; OTTHUMP00000229511 antibody; OTTHUMP00000229512 antibody; OTTHUMP00000229514 antibody; OTTHUMP00000229515 antibody; OTTHUMP00000229516 antibody; Preprocathepsin B antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase C1 family.

TISSUE SPECIFICITY

Expressed in the stratum spinosum of the epidermis. Weak expression is detected in the stratum granulosum.

SUBCELLULAR LOCATION

Secreted

FUNCTION

Cathepsin B is an enzymatic protein belonging to the peptidase (or protease) families. In humans, it is coded by the CTSB gene. A wide array of diseases results in elevated levels of cathepsin B, which causes numerous pathological processes including cell death, inflammation, and production of toxic peptides. Focusing on neurological diseases, cathepsin B gene knockout studies in an epileptic rodent model have shown cathepsin B causes a significant amount of the apoptotic cell death that occurs as a result of inducing epilepsy. Mutations in the CTSB gene have been linked to tropical pancreatitis, a form of chronic pancreatitis.