PRODUCT CODE: ET1704-03

Cathepsin B Recombinant Rabbit Monoclonal Antibody [JA11-02] (ET1704-03)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of Cathepsin B on different cells lysates using anti-Cathepsin B antibody at 1/500 dilution.<br />
Positive control: <br />
  Lane 1: HepG2 <br />
  Lane 2: Hela <br />
  Lane 3:A549
  • Western blot analysis of Cathepsin B on different cells lysates using anti-Cathepsin B antibody at 1/500 dilution.<br />
Positive control: <br />
  Lane 1: HepG2 <br />
  Lane 2: Hela <br />
  Lane 3:A549
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Cathepsin B antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cathepsin B antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Cathepsin B antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Cathepsin B antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Cathepsin B antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Cathepsin B antibody. Counter stained with hematoxylin.
Western blot analysis of Cathepsin B on different cells lysates using anti-Cathepsin B antibody at 1/500 dilution.
Positive control:
Lane 1: HepG2
Lane 2: Hela
Lane 3:A549

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Cathepsin B Recombinant Rabbit Monoclonal Antibody [JA11-02] (ET1704-03)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HepG2, A549, Hela, human liver tissue, human liver cancer tissue, human pancreas tissue, mouse pancreas tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA11-02

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

38 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Cathepsin B

SYNONYMS

Amyloid precursor protein secretase antibody; APP secretase antibody; APPS antibody; CATB_HUMAN antibody; Cathepsin B heavy chain antibody; Cathepsin B1 antibody; CathepsinB antibody; CPSB antibody; CTSB antibody; cysteine protease antibody; OTTHUMP00000116009 antibody; OTTHUMP00000229510 antibody; OTTHUMP00000229511 antibody; OTTHUMP00000229512 antibody; OTTHUMP00000229514 antibody; OTTHUMP00000229515 antibody; OTTHUMP00000229516 antibody; Preprocathepsin B antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase C1 family.

TISSUE SPECIFICITY

Expressed in the stratum spinosum of the epidermis. Weak expression is detected in the stratum granulosum.

SUBCELLULAR LOCATION

Lysosome. Melanosome.

FUNCTION

The cathepsin family of proteolytic enzymes contains several diverse classes of proteases. The cysteine protease class comprises cathepsins B, L, H, K, S, and O. The aspartyl protease class is composed of cathepsins D and E. Cathepsin G is in the serine protease class. Most cathepsins are lysosomal and each is involved in cellular metabolism, participating in various events such as peptide biosynthesis and protein degradation. Cathepsin B is expressed in luminal epithelial cells, indicating that cathepsin B is a marker for secretory cell death.