Cathepsin B Mouse Monoclonal Antibody [J11-A11] (M1506-1)
Catalog# M1506-1
Mouse Monoclonal to Cathepsin B
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WB
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IF-Cell
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Human
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Zebrafish
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Mouse
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Rat
Lane 1: SW480 cell lysate
Lane 2: RAW264.7 cell lysate
Lane 3: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 38 kDa
Observed band size: 38 kDa
Exposure time: 59 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1506-1) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
Cathepsin B Mouse Monoclonal Antibody [J11-A11] (M1506-1)
Immunogen
Synthetic peptide within human cathepsin b aa 300-338/338.
Host
Mouse
Positive Control
SW480 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, SW480, PC-12.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
J11-A11
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:50-1:500
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 38 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
Cathepsin B
SYNONYMS
Amyloid precursor protein secretase antibody;APP secretase antibody;APPS antibody;CATB_HUMAN antibody;Cathepsin B heavy chain antibody;Cathepsin B1 antibody;CathepsinB antibody;CPSB antibody;CTSB antibody;cysteine protease antibody;OTTHUMP00000116009 antibody;OTTHUMP00000229510 antibody;OTTHUMP00000229511 antibody;OTTHUMP00000229512 antibody;OTTHUMP00000229514 antibody;OTTHUMP00000229515 antibody;OTTHUMP00000229516 antibody;Preprocathepsin B antibody
SEQUENCE SIMILARITIES
Belongs to the peptidase C1 family.
TISSUE SPECIFICITY
Expressed in the stratum spinosum of the epidermis. Weak expression is detected in the stratum granulosum.
SUBCELLULAR LOCATION
Lysosome, Melanosome, Secreted, Apical cell membrane.
FUNCTION
Cathepsin B is an enzymatic protein belonging to the peptidase (or protease) families. In humans, it is coded by the CTSB gene. A wide array of diseases results in elevated levels of cathepsin B, which causes numerous pathological processes including cell death, inflammation, and production of toxic peptides. Focusing on neurological diseases, cathepsin B gene knockout studies in an epileptic rodent model have shown cathepsin B causes a significant amount of the apoptotic cell death that occurs as a result of inducing epilepsy. Mutations in the CTSB gene have been linked to tropical pancreatitis, a form of chronic pancreatitis
CITATIONS
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Elsaid, H. O. A., Tjeldnes, H., Rivedal, M., Serre, C., Eikrem, Ø., Svarstad, E., Tøndel, C., Marti, H. P., Furriol, J., & Babickova, J.
Gene Expression Analysis in gla-Mutant Zebrafish Reveals Enhanced Ca2+ Signaling Similar to Fabry Disease
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WB
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IHC
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Zebrafish
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Citation
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