PRODUCT CODE: M1506-1

Cathepsin B Mouse Monoclonal Antibody [J11-A11] (M1506-1)

  • Zebrafish

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Zebrafish

Western blot analysis of Cathepsin B on SW480 cell lysate using anti-Cathepsin B antibody at 1/5,000 dilution.
  • Western blot analysis of Cathepsin B on SW480 cell lysate using anti-Cathepsin B antibody at 1/5,000 dilution.
  • ICC staining Cathepsin B in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Cathepsin B (red) in HepG2 cells.  Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of Cathepsin B on SW480 cell lysate using anti-Cathepsin B antibody at 1/5,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Zebrafish

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Cathepsin B Mouse Monoclonal Antibody [J11-A11] (M1506-1)

Immunogen

Recombinant protein

Host

Mouse

Positive Control

SW480, HepG2

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

J11-A11

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

38 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:5,000-1:10,000

  • ICC

  • 1:200-1:500

TARGET

UNIPROT #

PROTEIN NAME

Cathepsin B

SYNONYMS

Amyloid precursor protein secretase antibody; APP secretase antibody; APPS antibody; CATB_HUMAN antibody; Cathepsin B heavy chain antibody; Cathepsin B1 antibody; CathepsinB antibody; CPSB antibody; CTSB antibody; cysteine protease antibody; OTTHUMP00000116009 antibody; OTTHUMP00000229510 antibody; OTTHUMP00000229511 antibody; OTTHUMP00000229512 antibody; OTTHUMP00000229514 antibody; OTTHUMP00000229515 antibody; OTTHUMP00000229516 antibody; Preprocathepsin B antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase C1 family.

TISSUE SPECIFICITY

Expressed in the stratum spinosum of the epidermis. Weak expression is detected in the stratum granulosum.

SUBCELLULAR LOCATION

Lysosome, secreted

FUNCTION

Cathepsin B is an enzymatic protein belonging to the peptidase (or protease) families. In humans, it is coded by the CTSB gene. A wide array of diseases results in elevated levels of cathepsin B, which causes numerous pathological processes including cell death, inflammation, and production of toxic peptides. Focusing on neurological diseases, cathepsin B gene knockout studies in an epileptic rodent model have shown cathepsin B causes a significant amount of the apoptotic cell death that occurs as a result of inducing epilepsy. Mutations in the CTSB gene have been linked to tropical pancreatitis, a form of chronic pancreatitis