Caspase-8 Recombinant Rabbit Monoclonal Antibody [SD08-06] (ET1612-70)

UNIPROT #

Q14790

PROTEIN NAME

Caspase-8

SYNONYMS

ALPS2B antibody;Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein antibody;Apoptotic cysteine protease antibody;Apoptotic protease Mch-5 antibody;Apoptotic protease Mch5 antibody;CAP4 antibody;CASP-8 antibody;CASP8 antibody;CASP8_HUMAN antibody;Caspase8;Caspase 8 antibody;Caspase 8 apoptosis related cysteine peptidase antibody;Caspase-8 subunit p10 antibody;CED 3 antibody;FADD Like ICE antibody;FADD-homologous ICE/CED-3-like protease antibody;FADD-like ICE antibody;FLICE antibody;FLJ17672 antibody;ICE-like apoptotic protease 5 antibody;MACH alpha 1/2/3 protein antibody;MACH antibody;MACH beta 1/2/3/4 protein antibody;MCH5 antibody;MGC78473 antibody;MORT1 associated ced 3 homolog antibody;MORT1-associated CED-3 homolog antibody;OTTHUMP00000163717 antibody;OTTHUMP00000163720 antibody;OTTHUMP00000163724 antibody;OTTHUMP00000163725 antibody;OTTHUMP00000165062 antibody;OTTHUMP00000165063 antibody;OTTHUMP00000165064 antibody;OTTHUMP00000206552 antibody;OTTHUMP00000206582 antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase C14A family.

TISSUE SPECIFICITY

Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.

POST-TRANSLATIONAL MODIFICATION

Generation of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events.; Phosphorylation on Ser-387 during mitosis by CDK1 inhibits activation by proteolysis and prevents apoptosis. This phosphorylation occurs in cancer cell lines, as well as in primary breast tissues and lymphocytes.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

Caspase-8 is a caspase protein, encoded by the CASP8 gene. It most likely acts upon caspase-3. CASP8 orthologs have been identified in numerous mammals for which complete genome data are available. These unique orthologs are also present in birds. The CASP8 gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.