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PRODUCT CODE: ER30804

Caspase-3 Rabbit Polyclonal Antibody (ER30804)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

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Western blot analysis of Caspase-3 on different lysates using anti- Caspase-3 antibody at 1/1,000 dilution.<br /> Positive control:<br /> Lane 1: Human lung<br /> Lane 2: Human brain<br /> Lane 3: Jurkat<br /> Lane 4: F9<br /> Lane 5: NCCTI<br /> Lane 6: Daudi<br /> Lane 7: Raji<br /> Lane 8: MCF-7<br /> Lane 9: Hela
  • Western blot analysis of Caspase-3 on different lysates using anti- Caspase-3 antibody at 1/1,000 dilution.<br /> Positive control:<br /> Lane 1: Human lung<br /> Lane 2: Human brain<br /> Lane 3: Jurkat<br /> Lane 4: F9<br /> Lane 5: NCCTI<br /> Lane 6: Daudi<br /> Lane 7: Raji<br /> Lane 8: MCF-7<br /> Lane 9: Hela
  • ICC staining of Caspase-3 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining of Caspase-3 in A549 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Caspase-3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Caspase-3 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with Caspase-3 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
Western blot analysis of Caspase-3 on different lysates using anti- Caspase-3 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Human lung
Lane 2: Human brain
Lane 3: Jurkat
Lane 4: F9
Lane 5: NCCTI
Lane 6: Daudi
Lane 7: Raji
Lane 8: MCF-7
Lane 9: Hela

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Caspase-3 Rabbit Polyclonal Antibody (ER30804)

Immunogen

Synthetic peptide within n-terminal human caspase-3.

Host

Rabbit

Positive Control

Jurkat, F9, NCCIT, Hela, Raji, MCF-7, Daudi, A549, human tosil tissue, human liver cancer tissue, human lung tissue,human brain tissue,HepG2

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

31 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:200

  • IHC-P

  • 1:200

  • FC

  • 1:100-1:200

TARGET

UNIPROT #

P42574

PROTEIN NAME

Caspase-3

SYNONYMS

A830040C14Rik antibody; Apopain antibody; CASP 3 antibody; CASP-3 antibody; CASP3 antibody; CASP3_HUMAN antibody; Casp3a antibody; Caspase 3 antibody; Caspase 3, apoptosis-related cysteine peptidase antibody; Caspase 3, apoptosis-related cysteine protease antibody; Caspase 3, apoptosis-related cysteine protease a antibody; Caspase-3 subunit p12 antibody; Caspase3 antibody; CC3 antibody; CPP 32 antibody; CPP-32 antibody; CPP32 antibody; CPP32B antibody; Cysteine protease CPP32 antibody; EC 3.4.22.56 antibody; ICE3 antibody; LICE antibody; mldy antibody; OTTHUMP00000165052 antibody; OTTHUMP00000165053 antibody; OTTHUMP00000165054 antibody; PARP cleavage protease antibody; Procaspase3 antibody; Protein Yama antibody; SCA 1 antibody; SCA-1 antibody; SCA1 antibody; SREBP cleavage activity 1 antibody; Yama antibody; Yama protein antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase C14A family.

TISSUE SPECIFICITY

Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.

POST-TRANSLATIONAL MODIFICATION

Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.; S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.

SUBCELLULAR LOCATION

Nucleus, cytoplasm,

FUNCTION

Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP). Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit.

CITATIONS

  • Liu, Junjun et al.

    Sini decoction alleviates E. coli induced acute lung injury in mice via equilibrating ACE-AngII-AT1R and ACE2-Ang-(1-7)-Mas axis. | Life Sciences [2018]

  • Chen, Qiuhua et al.

    Sini decoction ameliorates sepsis-induced acute lung injury via regulating ACE2-Ang (1-7)-Mas axis and inhibiting the MAPK signaling pathway. | Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie [2019]

  • Liu, Xia et al.

    Silencing RRM2 inhibits multiple myeloma by targeting the Wnt/β-catenin signaling pathway. | Molecular Medicine Reports [2019]

  • Chen, Fen et al.

    Combination of HSP90 and autophagy inhibitors promotes hepatocellular carcinoma apoptosis following incomplete thermal ablation. | Molecular Medicine Reports [2020]