Cleaved+pro Caspase-3 Recombinant Rabbit Monoclonal Antibody [JE75-05] (HA722367)
Catalog# HA722367
Rabbit Monoclonal to Caspase-3
-
WB
-
Human
-
Mouse
Western blot analysis of Cleaved+pro Caspase-3 on different lysates with Rabbit anti-Cleaved+pro Caspase-3 antibody (HA722367) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 1μM staurosporine for 4 hours cell lysate
Lane 3: NIH/3T3 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 32 kDa
Observed band size: 32/17 kDa
Exposure time: 2 minutes 7 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722367) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Cleaved+pro Caspase-3 Recombinant Rabbit Monoclonal Antibody [JE75-05] (HA722367)
Immunogen
Recombinant protein within human caspase-3 aa 1-200 / 277.
Host
Rabbit
Positive Control
HeLa cell lysate, HeLa treated with 1μM staurosporine for 4 hours cell lysate, NIH/3T3 cell lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE75-05
APPLICATION DILUTION
-
WB
-
1:1,000-1:2,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 32 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Caspase-3
SYNONYMS
Caspase3;A830040C14Rik antibody;Apopain antibody;CASP 3 antibody;CASP-3 antibody;CASP3 antibody;CASP3_HUMAN antibody;Casp3a antibody;Caspase 3 antibody;Caspase 3, apoptosis-related cysteine peptidase antibody;Caspase 3, apoptosis-related cysteine protease antibody;Caspase 3, apoptosis-related cysteine protease a antibody;Caspase-3 subunit p12 antibody;Caspase3 antibody;CC3 antibody;CPP 32 antibody;CPP-32 antibody;CPP32 antibody;CPP32B antibody;Cysteine protease CPP32 antibody;EC 3.4.22.56 antibody;ICE3 antibody;LICE antibody;mldy antibody;OTTHUMP00000165052 antibody;OTTHUMP00000165053 antibody;OTTHUMP00000165054 antibody;PARP cleavage protease antibody;Procaspase3 antibody;Protein Yama antibody;SCA 1 antibody;SCA-1 antibody;SCA1 antibody;SREBP cleavage activity 1 antibody;Yama antibody;Yama protein antibody
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
Caspase-3 is a caspase protein that interacts with caspase-8 and caspase-9. It is encoded by the CASP3 gene. CASP3 orthologs have been identified in numerous mammals for which complete genome data are available. Unique orthologs are also present in birds, lizards, lissamphibians, and teleosts. Caspase-3 shares many of the typical characteristics common to all currently-known caspases. For example, its active site contains a cysteine residue (Cys-163) and histidine residue (His-121) that stabilize the peptide bond cleavage of a protein sequence to the carboxy-terminal side of an aspartic acid when it is part of a particular 4-amino acid sequence. This specificity allows caspases to be incredibly selective, with a 20,000-fold preference for aspartic acid over glutamic acid. A key feature of caspases in the cell is that they are present as zymogens, termed procaspases, which are inactive until a biochemical change causes their activation. Each procaspase has an N-terminal large subunit of about 20 kDa followed by a smaller subunit of about 10 kDa, called p20 and p10, respectively.
CITATIONS
-
Zhang Hongya,et al
Mice with NOP2/sun RNA methyltransferase 5 deficiency die before reaching puberty due to fatal kidney damage
-
IHC
-
Mouse
-
Citation
-
