Caspase-1 + p10 + p12 Recombinant Rabbit Monoclonal Antibody [JE56-35] (HA722222)
Catalog# HA722222
Rabbit Monoclonal to Caspase-1 + p10 + p12
Tested for
-
WB
-
IF-Cell
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IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Caspase-1 + p10 + p12 on different lysates with Rabbit anti-Caspase-1 + p10 + p12 antibody (HA722222) at 1/2,000 dilution.
Lane 1: Raji cell lysate
Lane 2: THP-1 cell lysate
Lane 3: U-937 cell lysate
Lane 4: HepG2 cell lysate
Lane 5: RAW264.7 cell lysate
Lane 6: Mouse spleen tissue lysate
Lane 7: Rat spleen tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 45/42/35/12/10 kDa
Observed band size: 45/42/35/12/10 kDa
Exposure time: 1 minute 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722222) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Raji cell lysate
Lane 2: THP-1 cell lysate
Lane 3: U-937 cell lysate
Lane 4: HepG2 cell lysate
Lane 5: RAW264.7 cell lysate
Lane 6: Mouse spleen tissue lysate
Lane 7: Rat spleen tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 45/42/35/12/10 kDa
Observed band size: 45/42/35/12/10 kDa
Exposure time: 1 minute 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722222) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
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