Caspase-1 + p10 + p12 Recombinant Rabbit Monoclonal Antibody [JE56-35] (HA722222)
Catalog# HA722222
Rabbit Monoclonal to Caspase-1 + p10 + p12
Tested for
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WB
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IF-Cell
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IHC-P
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Caspase-1 + p10 + p12 on different lysates with Rabbit anti-Caspase-1 + p10 + p12 antibody (HA722222) at 1/5,000 dilution.
Lane 1: Raji cell lysate
Lane 2: THP-1 cell lysate
Lane 3: U-937 cell lysate
Lane 4: A549 cell lysate (low expression)
Lane 5: RAW264.7 cell lysate
Lane 6: Mouse spleen tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 45/42/35/12/10 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722222) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Raji cell lysate
Lane 2: THP-1 cell lysate
Lane 3: U-937 cell lysate
Lane 4: A549 cell lysate (low expression)
Lane 5: RAW264.7 cell lysate
Lane 6: Mouse spleen tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 45/42/35/12/10 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722222) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.