Caspase-1 Rabbit Polyclonal Antibody (R1510-23)
Catalog# R1510-23
Rabbit Polyclonal to Caspase-1
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IF-Cell
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IHC-P
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FC
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Human
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Caspase-1 antibody (R1510-23) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
Caspase-1 Rabbit Polyclonal Antibody (R1510-23)
Immunogen
Synthetic peptide within human caspase-1 aa 162-205.
Host
Rabbit
Positive Control
Jurkat, human lung tissue, human liver tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 45 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Caspase-1
SYNONYMS
Caspase1;CASP-1 antibody;CASP1 antibody;CASP1_HUMAN antibody;Caspase1 antibody;Caspase 1 antibody;Caspase-1 subunit p10 antibody;ICE antibody;IL-1 beta-converting enzyme antibody;IL-1BC antibody;IL1 beta converting enzyme antibody;IL1B convertase antibody;Interleukin 1 beta convertase antibody;Interleukin 1B converting enzyme antibody;Interleukin-1 beta convertase antibody;Interleukin-1 beta-converting enzyme antibody;p45 antibody
SEQUENCE SIMILARITIES
Belongs to the peptidase C14A family.
TISSUE SPECIFICITY
Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
POST-TRANSLATIONAL MODIFICATION
The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
Caspase-1, originally designated ICE (for IL-1 converting enzyme), is a member of the group of caspases with large prodomains. Caspase-1 promotes maturation of interleukin IL-1β and interleukin18 (IL-18) by proteolytic cleavage of precursor forms into biologically active pro-inflamatory cytokines. Active caspase-1, a (p20/p10)2 tetramer, is necessary and sufficient for cleavage of precursor IL-1 as well as for induction of apoptosis in some cell lines. The highly conserved family of caspases mediate many of the morphological and biochemical features of apoptosis, including structural dismantling of cell bodies and nuclei, fragmentation of genomic DNA, destruction of regulatory proteins and propagation of other pro-apoptotic molecules. The human Caspase-1 gene maps to chromosome 2q14 and encodes a cytoplasmic protein expressed in liver, heart, skeletal muscle kidney and testis. Caspase-1 has been implicated in inflammation, septic shock, and other situations such as wound healing and the growth of certain leukemias.
CITATIONS
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Cui, B., Liu, Y., Chen, J., Chen, H., Feng, Y., & Zhang, P.
Small molecule inhibitor CRT0066101 inhibits cytokine storm syndrome in a mouse model of lung injury
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IF
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Mouse
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Citation
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