CASK Mouse Monoclonal Antibody [A1C5] (EM1902-09)
Catalog# EM1902-09
Mouse Monoclonal to CASK
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
Knock-Out or Knock-Down validated
Western blot analysis of CASK on different lysates with Mouse anti-CASK antibody (EM1902-09) at 1/2,000 dilution.
Lane 1: SiHa cell lysate
Lane 2: A549 cell lysate
Lane 3: U-87 MG cell lysate
Lane 4: Neuro-2a cell lysate
Lane 5: Human brain tissue lysate
Lane 6: Mouse brain tissue lysate
Lane 7: Rat brain tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 105 kDa
Observed band size: 105 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-09) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: SiHa cell lysate
Lane 2: A549 cell lysate
Lane 3: U-87 MG cell lysate
Lane 4: Neuro-2a cell lysate
Lane 5: Human brain tissue lysate
Lane 6: Mouse brain tissue lysate
Lane 7: Rat brain tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 105 kDa
Observed band size: 105 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-09) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.