CARS Recombinant Rabbit Monoclonal Antibody [JE55-84] (ET7111-18)
Catalog# ET7111-18
Rabbit Monoclonal to CARS
Tested for
-
WB
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IHC-P
Reacts with
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Human
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of CARS on different lysates with Rabbit anti-CARS antibody (ET7111-18) at 1/5,000 dilution.
Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 85 kDa
Observed band size: 75 kDa
Exposure time: 59 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-18) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 85 kDa
Observed band size: 75 kDa
Exposure time: 59 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-18) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.