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PRODUCT CODE: EM1701-39

Cardiac Troponin T Mouse Monoclonal Antibody [3-G6] (EM1701-39)

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Mouse

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Western blot analysis of Cardiac Troponin T on human heart tissue lysate using anti- Cardiac Troponin T antibody at 1/1,000 dilution.
  • Western blot analysis of Cardiac Troponin T on human heart tissue lysate using anti- Cardiac Troponin T antibody at 1/1,000 dilution.
  • ICC staining Cardiac Troponin T (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Cardiac Troponin T (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti- Cardiac Troponin T antibody. Counter stained with hematoxylin.
Western blot analysis of Cardiac Troponin T on human heart tissue lysate using anti- Cardiac Troponin T antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Cardiac Troponin T Mouse Monoclonal Antibody [3-G6] (EM1701-39)

Immunogen

Peptide.

Host

Mouse

Positive Control

Human heart tissue lysate, Hela, A431, mouse heart tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

3-G6

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G purified.

MOLECULAR WEIGHT

36 kDa

Isotype

IgG2b

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

P45379

PROTEIN NAME

Cardiac Troponin T

SYNONYMS

Cardiac muscle troponin T antibody; Cardiomyopathy dilated 1D (autosomal dominant) antibody; Cardiomyopathy hypertrophic 2 antibody; CMD1D antibody; CMH2 antibody; CMPD2 antibody; cTnT antibody; LVNC6 antibody; MGC3889 antibody; OTTHUMP00000033864 antibody; OTTHUMP00000033865 antibody; OTTHUMP00000033866 antibody; OTTHUMP00000033867 antibody; OTTHUMP00000033870 antibody; OTTHUMP00000218095 antibody; RCM3 antibody; TNNT 2 antibody; TNNT2 antibody; TNNT2_HUMAN antibody; TnTc antibody; Troponin T cardiac muscle antibody; Troponin T type 2 (cardiac) antibody; Troponin T type 2 cardiac antibody; Troponin T, cardiac muscle antibody; Troponin T2 cardiac antibody

SEQUENCE SIMILARITIES

Belongs to the troponin T family.

TISSUE SPECIFICITY

Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Thr-213 by PRKCA induces significant reduction in myofilament calcium sensitivity and actomyosin ATPase activity.

SUBCELLULAR LOCATION

Cytoskeleton, Cytosol.

FUNCTION

Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2), cardiomyopathy dilated type 1D (CMD1D) and cardiomyopathy familial restrictive type 3 (RCM3).