Applications
-
WB
-
IHC-P
-
ICC
REACTIVITY
-
Human
-
Mouse
SPECIFICATIONS
Product Type
Mouse monoclonal primary
Product Name
Cardiac Troponin T Mouse Monoclonal Antibody [3-G6] (EM1701-39)
Immunogen
Synthetic peptide within huamn cardiac troponin t aa 45-89/298.
Host
Mouse
Positive Control
Human heart tissue lysate, Hela, A431, mouse heart tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
3-G6
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
PURIFICATION
Protein G affinity purified.
MOLECULAR WEIGHT
36 kDa
Isotype
IgG2b
APPLICATION DILUTION
-
WB
-
1:500-1:1,000
-
ICC
-
1:50-1:200
-
IHC-P
-
1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
Cardiac Troponin T
SYNONYMS
Cardiac muscle troponin T antibody;Cardiomyopathy dilated 1D (autosomal dominant) antibody;Cardiomyopathy hypertrophic 2 antibody;CMD1D antibody;CMH2 antibody;CMPD2 antibody;cTnT antibody;LVNC6 antibody;MGC3889 antibody;OTTHUMP00000033864 antibody;OTTHUMP00000033865 antibody;OTTHUMP00000033866 antibody;OTTHUMP00000033867 antibody;OTTHUMP00000033870 antibody;OTTHUMP00000218095 antibody;RCM3 antibody;TNNT 2 antibody;TNNT2 antibody;TNNT2_HUMAN antibody;TnTc antibody;Troponin T cardiac muscle antibody;Troponin T type 2 (cardiac) antibody;Troponin T type 2 cardiac antibody;Troponin T, cardiac muscle antibody;Troponin T2 cardiac antibody
SEQUENCE SIMILARITIES
Belongs to the troponin T family.
TISSUE SPECIFICITY
Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation at Thr-213 by PRKCA induces significant reduction in myofilament calcium sensitivity and actomyosin ATPase activity.
SUBCELLULAR LOCATION
Cytoskeleton, Cytosol.
FUNCTION
Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2), cardiomyopathy dilated type 1D (CMD1D) and cardiomyopathy familial restrictive type 3 (RCM3).