Carcino Embryonic Antigen CEA Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [JM93-28] (HA750434)
Catalog# HA750434
Rabbit Monoclonal to Carcino Embryonic Antigen CEA
Tested for
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WB
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IF-Cell
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IF-Tissue
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IHC-P
Reacts with
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Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Carcino Embryonic Antigen CEA on different lysates with Rabbit anti-Carcino Embryonic Antigen CEA antibody (HA750434) at 1/1,000 dilution.
Lane 1: A549 cell lysate
Lane 2: MCF7 cell lysate
Lane 3: BxPC-3 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 77 kDa
Observed band size: 70-200 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750434) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A549 cell lysate
Lane 2: MCF7 cell lysate
Lane 3: BxPC-3 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 77 kDa
Observed band size: 70-200 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750434) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.