PRODUCT CODE: ER1802-72

Carbonic anhydrase 2 Rabbit Polyclonal Antibody (ER1802-72)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of Carbonic anhydrase 2 on different cell lysates using anti-Carbonic anhydrase 2 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: 293 <br />
         Lane 2: THP-1<br />
 Lane 3: HL-60
  • Western blot analysis of Carbonic anhydrase 2 on different cell lysates using anti-Carbonic anhydrase 2 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: 293 <br />
         Lane 2: THP-1<br />
 Lane 3: HL-60
  • ICC staining Carbonic anhydrase 2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Carbonic anhydrase 2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Carbonic anhydrase 2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Carbonic anhydrase 2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Carbonic anhydrase 2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-Carbonic anhydrase 2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Carbonic anhydrase 2 antibody. Counter stained with hematoxylin.
Western blot analysis of Carbonic anhydrase 2 on different cell lysates using anti-Carbonic anhydrase 2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: 293
Lane 2: THP-1
Lane 3: HL-60

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Carbonic anhydrase 2 Rabbit Polyclonal Antibody (ER1802-72)

Immunogen

Recombinant protein within human carbonic anhydrase 2 aa 40-240.

Host

Rabbit

Positive Control

293, THP-1, HL-60, 293T, Hela, LOVO, human liver tissue, human colon cancer tissue, human appendix tissue, human stomach cancer tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

29 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Carbonic anhydrase 2

SYNONYMS

can antibody; CAN_ECOLI antibody; Carbonate dehydratase 2 antibody; Carbonic anhydrase 2 antibody

SEQUENCE SIMILARITIES

Belongs to the alpha-carbonic anhydrase family.

SUBCELLULAR LOCATION

Cytoplasm. Cell membrane.

FUNCTION

Carbonic anhydrases (CAs) are members of a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes including respiration, calcification, acid-base balance and bone resorption, as well as the formation of aqueous humor, cerebrospinal fluid, saliva and gastric juice. They show extensive diversity in distribution and in their subcellular localization. The human CA2 gene, which maps to chromosome 8q21, encodes CA II, a cytoplasmic protein that has the highest turnover rate and widest tissue distribution of any known human CA isozyme. The human CA4 gene, which maps to chromosome 17q23, encodes CA IV, a membrane-anchored isozyme that is expressed on the luminal surfaces of pulmonary capillaries and proximal renal tubules. The human CA9, CA12 and CA14 genes, which map to chromosomes 9p13, 15q22 and 1q21, respectively, encode transmembrane proteins that have unique patterns of tissue-specific expression. CA IX is specifically expressed in clear-cell renal carcinomas, whereas CA XII is highly expressed in normal tissues, such as kidney, colon and pancreas. Human CA XIV is also expressed in normal tissues, such as brain, but differs from CA XII in its expression pattern.