CAPZA1 Mouse Monoclonal Antibody [A2E7] (EM1901-82)
Catalog# EM1901-82
Mouse Monoclonal to CAPZA1
Tested for
-
WB
-
IF-Cell
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
Western blot analysis of CAPZA1 on different lysates with Mouse anti-CAPZA1 antibody (EM1901-82) at 1/5,000 dilution.
Lane 1: K-562 cell lysate
Lane 2: 293T cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HeLa cell lysate
Lane 5: HepG2 cell lysate
Lane 6: A549 cell lysate
Lane 7: NIH/3T3 cell lysate
Lane 8: RAW264.7 cell lysate
Lane 9: C2C12 cell lysate
Lane 10: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Exposure time: 46 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-82) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: K-562 cell lysate
Lane 2: 293T cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HeLa cell lysate
Lane 5: HepG2 cell lysate
Lane 6: A549 cell lysate
Lane 7: NIH/3T3 cell lysate
Lane 8: RAW264.7 cell lysate
Lane 9: C2C12 cell lysate
Lane 10: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Exposure time: 46 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-82) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.