PRODUCT CODE: ET1703-54

CaMKII alpha Recombinant Rabbit Monoclonal Antibody [JM11-07] (ET1703-54)

  • Recombinant

Applications

  • WB

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of CaMKII alpha on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-54, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of CaMKII alpha on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-54, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-CaMKII alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-54, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-CaMKII alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-54, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of CaMKII alpha was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1703-54, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of CaMKII alpha on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-54, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

CaMKII alpha Recombinant Rabbit Monoclonal Antibody [JM11-07] (ET1703-54)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

SH-SY5Y, rat brain cells.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM11-07

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

54 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • FC

  • 1:50-1:100

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

CaMKII alpha

SYNONYMS

Alpha CaMKII antibody; Calcium calmodulin dependent protein kinase II antibody; Calcium/calmodulin dependent protein kinase II alpha B subunit antibody; Calcium/calmodulin dependent protein kinase type II alpha chain antibody; Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha antibody; Calcium/calmodulin-dependent protein kinase II alpha antibody; Calcium/calmodulin-dependent protein kinase II-alpha antibody; Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody; Calcium/calmodulin-dependent protein kinase type IIA antibody; CaM kinase II alpha chain antibody; CaM kinase II alpha subunit antibody; CaM kinase II subunit alpha antibody; CaMK II alpha subunit antibody; CaMK-II subunit alpha antibody; Camk2a antibody; CAMKA antibody; CaMKII antibody; CaMKIINalpha antibody; EC 2.7.11.17 antibody; KCC2A_HUMAN antibody; KIAA0968 antibody; MGC123320 antibody; MGC139375 antibody; MGC155201 antibody; mKIAA0968 antibody; PK2CDD antibody; PKCCD antibody; R74975 antibody; zgc:112538 antibody; zgc:123320 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.

POST-TRANSLATIONAL MODIFICATION

Autophosphorylation of Thr-286 following activation by Ca(2+)/calmodulin. Phosphorylation of Thr-286 locks the kinase into an activated state.; Palmitoylated. Probably palmitoylated by ZDHHC3 and ZDHHC7.

SUBCELLULAR LOCATION

Cell junction, synapse, presynaptic cell membrane.

FUNCTION

The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is a ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes designated α, β, γ and δ, which may or may not be co-expressed in the same tissue type. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation. Stimulation of the T cell receptor CD3 signaling complex with an anti-CD3 monoclonal antibody leads to a 10-40 fold increase in CaMKIV activity. An additional kinase, CaMKK, functions to activate CaMKI through the specific phosphorylation of the regulatory Threonine residue at position 177.