Applications
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WB
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IHC-P
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ICC
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FC
REACTIVITY
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Human
SPECIFICATIONS
Product Type
Mouse monoclonal primary
Product Name
Calreticulin Mouse Monoclonal Antibody [7B2] (EM1701-61)
Immunogen
Recombinant protein.
Host
Mouse
Positive Control
HL-60, A431, HepG2, human thyroid gland tissue, human prostate cancer tissue, human placenta tissue, Jurkat.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
7B2
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 ug/ul
PURIFICATION
Protein G purified.
MOLECULAR WEIGHT
55 kDa
Isotype
IgG1
APPLICATION DILUTION
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WB
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1:500-1:2,000
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ICC
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
Calreticulin
SYNONYMS
Autoantigen RO antibody; CALR antibody; CALR protein antibody; CALR_HUMAN antibody; Calregulin antibody; Calreticulin antibody; cC1qR antibody; CRP55 antibody; CRT antibody; CRTC antibody; Endoplasmic reticulum resident protein 60 antibody; Epididymis secretory sperm binding protein Li 99n antibody; ERp60 antibody; FLJ26680 antibody; grp60 antibody; HACBP antibody; HEL S 99n antibody; RO antibody; Sicca syndrome antigen A (autoantigen Ro; calreticulin) antibody; Sicca syndrome antigen A antibody; SSA antibody
SEQUENCE SIMILARITIES
Belongs to the calreticulin family.
SUBCELLULAR LOCATION
Endoplasmic reticulum. Secreted. Cytosol.
FUNCTION
Calnexin and calregulin (also called calreticulin) are calcium-binding proteins that are localized to the endoplasmic reticulum, Calnexin to the membrane and calregulin to the lumen. Calnexin is a type I membrane protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may play a role in assisting with protein assembly and in retaining unassembled protein subunits in the endoplasmic reticulum. Calregulin has both low- and high-affinity calcium-binding sites. Neither Calnexin nor calregulin contains the calcium-binding "E-F hand" motif found in calmodulins. Calnexin and calregulin are important for the maturation of glycoproteins in the endoplasmic reticulum and appear to bind many of the same proteins.
CITATIONS
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Yuling Mi; Caiqiao Zhang
The Attenuating Effect of the Intraovarian Bone Morphogenetic Protein 4 on Age-Related Endoplasmic Reticulum Stress in Chicken Follicular Cells
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WB
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IHC
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IF
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hen
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Citation
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