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PRODUCT CODE: EM1701-61

Calreticulin Mouse Monoclonal Antibody [7B2] (EM1701-61)

Applications

  • WB

  • IHC-P

  • ICC

  • FC

REACTIVITY

  • Human

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Western blot analysis of Calreticulin on HL-60 (1) and A431 (2) cell lysates using anti-Calreticulin antibody at 1/1,000 dilution.
  • Western blot analysis of Calreticulin on HL-60 (1) and A431 (2) cell lysates using anti-Calreticulin antibody at 1/1,000 dilution.
  • ICC staining Calreticulin (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Jurkat cells with Calreticulin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
Western blot analysis of Calreticulin on HL-60 (1) and A431 (2) cell lysates using anti-Calreticulin antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • ICC

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Calreticulin Mouse Monoclonal Antibody [7B2] (EM1701-61)

Immunogen

Recombinant protein.

Host

Mouse

Positive Control

HL-60, A431, HepG2, human thyroid gland tissue, human prostate cancer tissue, human placenta tissue, Jurkat.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

7B2

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G purified.

MOLECULAR WEIGHT

55 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

P27797

PROTEIN NAME

Calreticulin

SYNONYMS

Autoantigen RO antibody; CALR antibody; CALR protein antibody; CALR_HUMAN antibody; Calregulin antibody; Calreticulin antibody; cC1qR antibody; CRP55 antibody; CRT antibody; CRTC antibody; Endoplasmic reticulum resident protein 60 antibody; Epididymis secretory sperm binding protein Li 99n antibody; ERp60 antibody; FLJ26680 antibody; grp60 antibody; HACBP antibody; HEL S 99n antibody; RO antibody; Sicca syndrome antigen A (autoantigen Ro; calreticulin) antibody; Sicca syndrome antigen A antibody; SSA antibody

SEQUENCE SIMILARITIES

Belongs to the calreticulin family.

SUBCELLULAR LOCATION

Endoplasmic reticulum. Secreted. Cytosol.

FUNCTION

Calnexin and calregulin (also called calreticulin) are calcium-binding proteins that are localized to the endoplasmic reticulum, Calnexin to the membrane and calregulin to the lumen. Calnexin is a type I membrane protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may play a role in assisting with protein assembly and in retaining unassembled protein subunits in the endoplasmic reticulum. Calregulin has both low- and high-affinity calcium-binding sites. Neither Calnexin nor calregulin contains the calcium-binding "E-F hand" motif found in calmodulins. Calnexin and calregulin are important for the maturation of glycoproteins in the endoplasmic reticulum and appear to bind many of the same proteins.

CITATIONS

  • Yuling Mi; Caiqiao Zhang

    The Attenuating Effect of the Intraovarian Bone Morphogenetic Protein 4 on Age-Related Endoplasmic Reticulum Stress in Chicken Follicular Cells