Calpain small subunit 1 Recombinant Rabbit Monoclonal Antibody [JE47-72] (ET7109-66)
Catalog# ET7109-66
Rabbit Monoclonal to Calpain small subunit 1
Tested for
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WB
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IHC-P
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FC
Reacts with
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Human
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Calpain small subunit 1 on different lysates with Rabbit anti-Calpain small subunit 1 antibody (ET7109-66) at 1/1,000 dilution.
Lane 1: 293T cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (20 µg/Lane)
Lane 3: Mouse lung tissue lysate (40 µg/Lane)
Lane 4: Rat lung tissue lysate (40 µg/Lane)
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 1 minute 40 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-66) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (20 µg/Lane)
Lane 3: Mouse lung tissue lysate (40 µg/Lane)
Lane 4: Rat lung tissue lysate (40 µg/Lane)
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 1 minute 40 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-66) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.