Calpain 2 Recombinant Rabbit Monoclonal Antibody [JA43-41] (ET1704-90)
Catalog# ET1704-90
Rabbit Monoclonal to Calpain 2
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Rat
Western blot analysis of Calpain 2 on different lysates with Rabbit anti-Calpain 2 antibody (ET1704-90) at 1/1,000 dilution.
Lane 1: HCT 116-si NT cell lysate
Lane 2: HCT 116-si Calpain 2 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 80 kDa
Observed band size: 75 kDa
Exposure time: 40 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-90) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Calpain 2 Recombinant Rabbit Monoclonal Antibody [JA43-41] (ET1704-90)
Immunogen
Synthetic peptide within human calpain 2 aa 651-700 / 700.
Host
Rabbit
Positive Control
HCT 116 cell lysate, MDA-MB-231 cell lysate, 293T cell lysate, HepG2, human lung tissue, human kidney tissue, Hela.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JA43-41
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IF-Cell
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1:50
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IHC-P
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1:50-1:200
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FC
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1:50
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 80 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Calpain 2
SYNONYMS
Calcium activated neutral proteinase 2 antibody;Calcium activated neutral proteinase antibody;Calcium-activated neutral protease 2, catalytic subunit antibody;Calcium-activated neutral proteinase 2 antibody;CALP80 antibody;Calpain 2 (m/II) large subunit antibody;Calpain 2 catalytic subunit antibody;Calpain 2 large catalytic subunit antibody;Calpain 2 large subunit antibody;Calpain 2, large [catalytic] subunit antibody;Calpain large polypeptide L2 antibody;Calpain M type antibody;Calpain M-type antibody;Calpain-2 catalytic subunit antibody;Calpain-2 large subunit antibody;Calpain2 antibody;CAN2_HUMAN antibody;CANP 2 antibody;CANP L2 antibody;CANP2 antibody;CANPL 2 antibody;CANPL2 antibody;CANPml antibody;Capa2 antibody;CAPN 2 antibody;CAPN2 antibody;FLJ39928 antibody;M calpain antibody;M calpin antibody;M type antibody;M-calpain antibody;mCANP antibody;Millimolar calpain antibody;Millimolar-calpain antibody
SEQUENCE SIMILARITIES
Belongs to the peptidase C2 family.
TISSUE SPECIFICITY
Ubiquitous.
SUBCELLULAR LOCATION
Cytoplasm. Cell membrane.
FUNCTION
Calpain-2 is a heterodimer of a catalytic subunit encoded by CAPN2 gene and a regulatory subunit CAPNS1. The catalytic subunit consists of four domains: protease core 1 domain (PC1), protease core 2 domain (PC2), calpain-type beta-sandwich-like domain (CBSW), and penta EF-hand domain (PEF(L)). The catalytic cleft is formed by PC1 and PC2 upon calcium binding. The catalytic triad consists of residues C105, H262, and N286. Noteworthy, CAPN2 also contains an N-terminal anchor helix, which however is cleaved off upon protease activation. There is no known consensus sequence for calpain-2 proteolysis, but there is evidence for over 130 potential substrates. Proteolytic cleavage by calpain-2 is regulated by presence of Ca2+ ions. It requires supraphysiological (low millimolar) concentration of Ca2+ for activation. Intracellular concentration of Ca2+ (approx. 100 nM) is insufficient for activating calpain-2, so activation occurs upon influx of ions from extracellular space or from endoplasmic reticulum.
CITATIONS
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Wu, H., Guo, H., Liu, H., Cui, H., Fang, J., Zuo, Z., Deng, J., Li, Y., Wang, X., & Zhao, L.
Copper sulfate-induced endoplasmic reticulum stress promotes hepatic apoptosis by activating CHOP, JNK and caspase-12 signaling pathways
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WB
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Mouse
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Citation
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