Calpain 1 Rabbit Polyclonal Antibody (R1701-3)
Catalog# R1701-3
Rabbit Polyclonal to Calpain 1
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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Rat
Specifications
Product Type
Rabbit polyclonal primary
Product Name
Calpain 1 Rabbit Polyclonal Antibody (R1701-3)
Immunogen
Recombinant protein within human calpain 1 aa 293-519 / 714.
Host
Rabbit
Positive Control
HUVEC, MCF-7, human tonsil tissue, human kidney tissue, 2 kidney tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:500
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 82 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Calpain 1
SYNONYMS
Ca2 activated neutral protease antibody;Calcium activated neutral proteinase antibody;Calcium activated neutral proteinase small subunit antibody;Calcium dependent protease small subunit 1 antibody;Calcium dependent protease small subunit antibody;Calcium-activated neutral proteinase 1 antibody;Calpain 1 antibody;Calpain 1 large subunit antibody;Calpain 1, (mu/I) large subunit antibody;Calpain mu type antibody;Calpain mu-type antibody;Calpain regulatory subunit antibody;Calpain small subunit 1 antibody;Calpain, large polypeptide L1 antibody;Calpain-1 catalytic subunit antibody;Calpain-1 large subunit antibody;CAN1_HUMAN antibody;CANP 1 antibody;CANP antibody;CANP small subunit antibody;CANP1 antibody;CANPL 1 antibody;CANPL1 antibody;CAPN 1 antibody;CAPN1 antibody;Cell proliferation inducing protein 30 antibody;Cell proliferation-inducing gene 30 protein antibody;Micromolar Calpain antibody;Micromolar-calpain antibody;Mu Calpain antibody;muCANP antibody;muCL antibody
SEQUENCE SIMILARITIES
Belongs to the peptidase C2 family.
TISSUE SPECIFICITY
Ubiquitous.
POST-TRANSLATIONAL MODIFICATION
Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms.
SUBCELLULAR LOCATION
Cytoplasm. Cell membrane.
FUNCTION
Calpain 1, also designated ?-calpain, is an intracellular calcium-dependent protease that cleaves cyto-skeletal and submembranous proteins. Calpains are nonlysosomal, calcium-activated intracellular cysteine proteases. Calpains mediate specific Ca2+-dependent processes including cell fusion, mitosis and meiosis. Calpains are heterodimers of a small regulatory subunit and one of three large catalytic subunits, designated Calpain 1, Calpain 2 and Calpain p94. Calpastatin regulates Calpain by inhibiting both the proteolytic activity of Calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing. Calpain-1 co-localizes with human leukocyte antigen-DR (HLA-DR) on activated microglia in the aging brain. Calpain influences the process of spermatogenesis and the events preceding fertilization, such as the acrosome reaction.
CITATIONS
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Liangen Shi
Ethanolic extract of Cordyceps cicadae exerts antitumor effect on human gastric cancer SGC-7901 cells by inducing apoptosis,cell cycle arrest and endoplasmic reticulum stress.
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WB
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Human
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Citation
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Mycobacterium tuberculosis PE_PGRS1 promotes mycobacteria intracellular survival via reducing the concentration of intracellular free Ca2+ and suppressing endoplasmic reticulum stress
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WB
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Human
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Citation
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