PRODUCT CODE: ER1803-42

Calnexin Rabbit Polyclonal Antibody (ER1803-42)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of Calnexin on mouse lung tissue (1) and Daudi cell (2) lysate using anti-Calnexin antibody at 1/500 dilution.
  • Western blot analysis of Calnexin on mouse lung tissue (1) and Daudi cell (2) lysate using anti-Calnexin antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Calnexin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Calnexin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Calnexin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calnexin antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SKOV-3 cells with Calnexin antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Calnexin on mouse lung tissue (1) and Daudi cell (2) lysate using anti-Calnexin antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Calnexin Rabbit Polyclonal Antibody (ER1803-42)

Immunogen

Synthetic peptide within c-terminal residues of rat calnexin.

Host

Rabbit

Positive Control

Daudi, mouse lung tissue, human colon tissue, human breast cancer tissue, human kidney tissue, mouse testis tissue, SKOV-3.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

90 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Calnexin

SYNONYMS

Calnexin antibody; CALX_HUMAN antibody; CANX antibody; CNX antibody; FLJ26570 antibody; Histocompatibility complex class I antigen binding protein p88 antibody; IP90 antibody; Major histocompatibility complex class I antigen-binding protein p88 antibody; p90 antibody

SEQUENCE SIMILARITIES

Belongs to the calreticulin family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated at Ser-564 by MAPK3/ERK1. phosphorylation by MAPK3/ERK1 increases its association with ribosomes (By similarity).; Palmitoylation by DHHC6 leads to the preferential localization to the perinuclear rough ER. It mediates the association of calnexin with the ribosome-translocon complex (RTC) which is required for efficient folding of glycosylated proteins.; Ubiquitinated, leading to proteasomal degradation. Probably ubiquitinated by ZNRF4.

SUBCELLULAR LOCATION

Endoplasmic reticulum.

FUNCTION

Calnexin and Calregulin (also called calreticulin) are calcium-binding proteins that are localized to the endoplasmic reticulum, Calnexin to the membrane and Calregulin to the lumen. Calnexin is a type I membrane protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may play a role in assisting with protein assembly and in retaining unassembled protein subunits in the endoplasmic reticulum. Calregulin has both low- and high-affinity calcium-binding sites. Neither Calnexin nor Calregulin contains the calcium-binding “E-F hand” motif found in calmodulins. Calnexin and Calregulin are important for the maturation of glycoproteins in the endoplasmic reticulum and appear to bind many of the same proteins.