Calnexin Rabbit Polyclonal Antibody (ER1803-42)
Catalog# ER1803-42
Rabbit Polyclonal to Calnexin
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WB
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IHC-P
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FC
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IP
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Human
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Mouse
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Rat
Lane 1: Daudi cell lysate (10 µg/Lane)
Lane 2: Mouse lung tissue lysate (20 µg/Lane)
Predicted band size: 68 kDa
Observed band size: 90 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-42) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/300,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
Calnexin Rabbit Polyclonal Antibody (ER1803-42)
Immunogen
Synthetic peptide within human calnexin aa 555-592/592.
Host
Rabbit
Positive Control
Daudi cell lysate, mouse lung tissue lysate, human kidney tissue, SKOV-3, Hela cell lysate, rat lung tissue lysate, rat brain tissue lysate, rat brain tissue, human brain tissue, mouse brain tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB:1:2,000- 1:10,000
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IHC-P
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1:200-1:500
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FC
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1:50-1:100
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IP
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1-2μg/sample
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 68 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Calnexin
SYNONYMS
Calnexin antibody;CALX_HUMAN antibody;CANX antibody;CNX antibody;FLJ26570 antibody;Histocompatibility complex class I antigen binding protein p88 antibody;IP90 antibody;Major histocompatibility complex class I antigen-binding protein p88 antibody;p90 antibody
SEQUENCE SIMILARITIES
Belongs to the calreticulin family.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated at Ser-564 by MAPK3/ERK1. phosphorylation by MAPK3/ERK1 increases its association with ribosomes (By similarity).; Palmitoylation by DHHC6 leads to the preferential localization to the perinuclear rough ER. It mediates the association of calnexin with the ribosome-translocon complex (RTC) which is required for efficient folding of glycosylated proteins.; Ubiquitinated, leading to proteasomal degradation. Probably ubiquitinated by ZNRF4.
SUBCELLULAR LOCATION
Endoplasmic reticulum membrane, Endoplasmic reticulum, Melanosome.
FUNCTION
Calnexin and Calregulin (also called calreticulin) are calcium-binding proteins that are localized to the endoplasmic reticulum, Calnexin to the membrane and Calregulin to the lumen. Calnexin is a type I membrane protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may play a role in assisting with protein assembly and in retaining unassembled protein subunits in the endoplasmic reticulum. Calregulin has both low- and high-affinity calcium-binding sites. Neither Calnexin nor Calregulin contains the calcium-binding “E-F hand” motif found in calmodulins. Calnexin and Calregulin are important for the maturation of glycoproteins in the endoplasmic reticulum and appear to bind many of the same proteins.
CITATIONS
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Yang Yuling,et al
Exosome/antimicrobial peptide laden hydrogel wound dressings promote scarless wound healing through miR-21-5p-mediated multiple functions
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WB
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Human
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Mouse
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Citation
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