PRODUCT CODE: ET1606-46

Calmodulin Recombinant Rabbit Monoclonal Antibody [SJ16-09] (ET1606-46)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Calmodulin on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1606-46, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of Calmodulin on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1606-46, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • ICC staining of Calmodulin in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1606-46, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of Calmodulin in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1606-46, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of Calmodulin in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1606-46, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Calmodulin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-46, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-Calmodulin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-46, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of Calmodulin on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1606-46, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Calmodulin Recombinant Rabbit Monoclonal Antibody [SJ16-09] (ET1606-46)

Immunogen

Synthetic peptide within human calmodulin aa 100-149 / 149.

Host

Rabbit

Positive Control

Rat brain tissue lysates, Hela, MCF-7, NIH/3T3, mouse brain tissue, mouse cerebellum tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SJ16-09

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

17 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Calmodulin

SYNONYMS

CALM 1 antibody; CALM 2 antibody; CALM 3 antibody; CALM antibody; CALM_HUMAN antibody; CALM1 antibody; CALM2 antibody; Calm3 antibody; CALML2 antibody; calmodulin 1 (phosphorylase kinase, delta) antibody; Calmodulin 2 (phosphorylase kinase, delta) antibody; Calmodulin 3 (phosphorylase kinase, delta) antibody; Calmodulin antibody; CaM antibody; CAM I antibody; CAM1 antibody; CAM2 antibody; CAM3 antibody; CAMB antibody; CAMC antibody; CAMI antibody; CAMII antibody; CPVT4 antibody; DD132 antibody; FLJ99410 antibody; LP7057 protein antibody; PHKD antibody; PHKD2 antibody; PHKD3 antibody; phosphorylase kinase delta antibody; phosphorylase kinase, delta subunit antibody

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

The level of intracellular calcium is tightly regulated in all eukaryotic cells. A modest increase in this level can result in a myriad of physiological responses, most of which are mediated by calmodulin (CaM), the universal calcium sensor. CaM directly modulates the activity of protein kinases and phosphatases, ion channels and nitric oxide synthetases. It is generally involved in such diverse processes as cell proliferation, endocytosis, cellular adhesion, protein turn over and smooth muscle contraction. CaM (calmodulin) is an acidic protein, 148 amino acids in length, with four helix-loop-helix calcium binding domains. In humans, 3 distinct genes have been identified (CALM1, CALM2 and CALM3); each encoding the identical protein. CALML3 (calmodulin-like 3, or calmodulin-related protein NB-1) shares significant sequence identity with CaM and it is suggested that it may competitively bind CaM substrates. Interestingly, CaM has been shown to associate with the carboxy terminus of the dystrophin gene product, implying that it may regulate its activity.