PRODUCT CODE: ER1803-38

CACNG3 Rabbit Polyclonal Antibody (ER1803-38)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

Western blot analysis of CACNG3 on SH-SY-5Y cell lysates using anti-CACNG3 antibody.<br />
  Lane 1: Anti-CACNG3 Antibody (1:500). <br />
  Lane 2: Anti-CACNG3 Antibody, pre-incubated with the immunizaiton peptide.
  • Western blot analysis of CACNG3 on SH-SY-5Y cell lysates using anti-CACNG3 antibody.<br />
  Lane 1: Anti-CACNG3 Antibody (1:500). <br />
  Lane 2: Anti-CACNG3 Antibody, pre-incubated with the immunizaiton peptide.
  • ICC staining CACNG3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CACNG3 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY-5Y cells with CACNG3 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of CACNG3 on SH-SY-5Y cell lysates using anti-CACNG3 antibody.
Lane 1: Anti-CACNG3 Antibody (1:500).
Lane 2: Anti-CACNG3 Antibody, pre-incubated with the immunizaiton peptide.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CACNG3 Rabbit Polyclonal Antibody (ER1803-38)

Immunogen

Synthetic peptide within rat cacng3 aa 200-250.

Host

Rabbit

Positive Control

SH-SY-5Y, rat brain tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

Predicted band size 36 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

CACNG3

SYNONYMS

CACNG 3 antibody; Cacng2 antibody; CACNG3 antibody; Calcium channel voltage dependent gamma subunit 3 antibody; CCG3_HUMAN antibody; Neuronal voltage gated calcium channel gamma 3 subunit antibody; Neuronal voltage-gated calcium channel gamma-3 subunit antibody; TARP gamma-3 antibody; Transmembrane AMPAR regulatory protein gamma-3 antibody; Voltage dependent calcium channel gamma 3 subunit antibody; Voltage gated calcium channel gamma subunit antibody; Voltage-dependent calcium channel gamma-3 subunit antibody

SEQUENCE SIMILARITIES

Belongs to the PMP-22/EMP/MP20 family. CACNG subfamily.

SUBCELLULAR LOCATION

Membrane. Displays a somatodendritic localization and is excluded from axons in neurons.

FUNCTION

Regulates the trafficking to the somatodendritic compartment and gating properties of AMPA-selective glutamate receptors (AMPARs). Promotes their targeting to the cell membrane and synapses and modulates their gating properties by slowing their rates of activation, deactivation and desensitization. Does not show subunit-specific AMPA receptor regulation and regulates all AMPAR subunits. Thought to stabilize the calcium channel in an inactivated (closed) state.