PRODUCT CODE: ER1901-89

CACNG2 Rabbit Polyclonal Antibody (ER1901-89)

Applications

  • WB

  • IHC-P

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of CACNG2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-89, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Mouse cerebellum tissue lysate<br />
Lane 2: Mouse brain tissue lysate<br />
Lane 3: Rat brain tissue lysate<br />
Predicted band size: 36 kDa<br />
Observed band size: 41 kDa
  • Western blot analysis of CACNG2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-89, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Mouse cerebellum tissue lysate<br />
Lane 2: Mouse brain tissue lysate<br />
Lane 3: Rat brain tissue lysate<br />
Predicted band size: 36 kDa<br />
Observed band size: 41 kDa
  • ICC staining of CACNG2 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-89, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of CACNG2 in SHSY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-89, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CACNG2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-89, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded rat Cerebellum tissue using anti-CACNG2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-89, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of CACNG2 was done on SHSY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-89, 1/100) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow).
Western blot analysis of CACNG2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-89, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse cerebellum tissue lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Rat brain tissue lysate
Predicted band size: 36 kDa
Observed band size: 41 kDa

Applications

  • WB

  • IHC-P

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CACNG2 Rabbit Polyclonal Antibody (ER1901-89)

Immunogen

Synthetic peptide within human cacng2 aa 196-245 / 323.

Host

Rabbit

Positive Control

Mouse cerebellum tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, A549, SHSY5Y, mouse brain tissue, rat Cerebellum tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

36 kDa (Predicted band size)

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2000

  • IHC-P

  • 1

  • 50-1:200

  • ICC

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

CACNG2

SYNONYMS

AW060990 antibody; B230105C07Rik antibody; B930041E13Rik antibody; Cacng2 antibody; Calcium channel voltage dependent gamma subunit 2 antibody; CaV gamma 2 antibody; CCG2_HUMAN antibody; Ipr328 antibody; MGC123981 antibody; MGC138502 antibody; MGC138504 antibody; Neuronal voltage gated calcium channel gamma 2 subunit antibody; Neuronal voltage-gated calcium channel gamma-2 subunit antibody; Stargazer antibody; stg antibody; TARP gamma-2 antibody; Transmembrane AMPAR regulatory protein gamma-2 antibody; Voltage dependent calcium channel gamma 2 subunit antibody; Voltage-dependent calcium channel gamma-2 subunit antibody; Wag antibody; Waggler antibody

SEQUENCE SIMILARITIES

Belongs to the PMP-22/EMP/MP20 family. CACNG subfamily.

TISSUE SPECIFICITY

Brain.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation of Thr-321 impairs interaction with DLG1 and DLG4.

SUBCELLULAR LOCATION

Cell junction, Membrane.

FUNCTION

Calcium channel, voltage-dependent, gamma subunit 2, also known as CACNG2 or stargazin is a protein that in humans is encoded by the CACNG2 gene. L-type calcium channels are composed of five subunits. The protein encoded by this gene represents one of these subunits, gamma, and is one of several gamma subunit proteins. It is an integral membrane protein that is thought to stabilize the calcium channel in an inactive (closed) state. This protein is similar to the mouse stargazin protein, mutations in which having been associated with absence seizures, also known as petit-mal or spike-wave seizures. This gene is a member of the neuronal calcium channel gamma subunit gene subfamily of the PMP-22/EMP/MP20 family. Stargazin is involved in the transportation of AMPA receptors to the synaptic membrane, and the regulation of their receptor rate constants — via its extracellular domain — once it is there. As it is highly expressed throughout the cerebral cortex, it is likely to have an important role in learning within these areas, due to the importance of AMPA receptors in LTP.