PRODUCT CODE: ER1803-49

CACNA1C Rabbit Polyclonal Antibody (ER1803-49)

Applications

  • Dot-blot

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Dot blot analysis of anti-CACNA1C on PVDF. 1ug, 2ug and 4ug of immunization peptides were given in this test. Anti-CACNA1C antibody was diluted with 1/500.
  • Dot blot analysis of anti-CACNA1C on PVDF. 1ug, 2ug and 4ug of immunization peptides were given in this test. Anti-CACNA1C antibody was diluted with 1/500.
  • ICC staining CACNA1C in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CACNA1C antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CACNA1C antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-CACNA1C antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-CACNA1C antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SKOV-3 cells with CACNA1C antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Dot blot analysis of anti-CACNA1C on PVDF. 1ug, 2ug and 4ug of immunization peptides were given in this test. Anti-CACNA1C antibody was diluted with 1/500.

Applications

  • Dot-blot

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CACNA1C Rabbit Polyclonal Antibody (ER1803-49)

Immunogen

Synthetic peptide within human cacna1c aa 800-880.

Host

Rabbit

Positive Control

SKOV-3, rat brain tissue, human kidney tissue, human uterus tissue, mouse heart tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

249 kDa

Isotype

IgG

APPLICATION DILUTION

  • Dot-blot

  • 1:500-1:1,000

  • ICC

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

CACNA1C

SYNONYMS

alpha-1 polypeptide antibody; cardiac muscle antibody; isoform 1 antibody; L type antibody; CAC1C_HUMAN antibody; CACH 2 antibody; CACH2 antibody; CACN 2 antibody; CACN2 antibody; CACNA1C antibody; CACNL1A1 antibody; Calcium channel antibody; Calcium channel cardic dihydropyridine sensitive alpha 1 subunit antibody; Calcium channel L type alpha 1 polypeptide isoform 1 cardiac muscle antibody; Calcium channel voltage dependent L type alpha 1C subunit antibody; CaV1.2 antibody; CCHL1A1 antibody; DHPR alpha 1 antibody; DHPR alpha 1 subunit antibody; LQT8 antibody; TS antibody; Voltage dependent L type calcium channel alpha 1C subunit antibody; Voltage dependent L type calcium channel subunit alpha 1C antibody; Voltage gated calcium channel alpha subunit Cav1.2 antibody; Voltage gated calcium channel subunit alpha Cav1.2 antibody; Voltage gated L type calcium channel Cav1.2 alpha 1 subunit, splice variant 10* antibody; Voltage-dependent L-type calcium channel subunit alpha-1C antibody; Voltage-gated calcium channel subunit alpha Cav1.2 antibody

SEQUENCE SIMILARITIES

Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1C subfamily.

TISSUE SPECIFICITY

Detected throughout the brain, including hippocampus, cerebellum and amygdala, throughout the heart and vascular system, including ductus arteriosus, in urinary bladder, and in retina and sclera in the eye. Expressed in brain, heart, jejunum, ovary, pancreatic beta-cells and vascular smooth muscle. Overall expression is reduced in atherosclerotic vascular smooth muscle.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation by PKA activates the channel. Elevated levels of blood glucose lead to increased phosphorylation by PKA.

SUBCELLULAR LOCATION

Plasma membrane.

FUNCTION

Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents. Mediates influx of calcium ions into the cytoplasm, and thereby triggers calcium release from the sarcoplasm. Plays an important role in excitation-contraction coupling in the heart. Required for normal heart development and normal regulation of heart rhythm. Required for normal contraction of smooth muscle cells in blood vessels and in the intestine. Essential for normal blood pressure regulation via its role in the contraction of arterial smooth muscle cells. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group (Probable).