PRODUCT CODE: M1009-3

C14orf93 Mouse Monoclonal Antibody [2-F6] (M1009-3)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

Western blot analysis of C14orf93 on C14orf93-GST recombinant protein lysate using anti-C14orf93 antibody at 1/1,000 dilution.
  • Western blot analysis of C14orf93 on C14orf93-GST recombinant protein lysate using anti-C14orf93 antibody at 1/1,000 dilution.
  • ICC staining C14orf93 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-C14orf93 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-C14orf93 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-C14orf93 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY5Y cells with C14orf93 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of C14orf93 on C14orf93-GST recombinant protein lysate using anti-C14orf93 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

C14orf93 Mouse Monoclonal Antibody [2-F6] (M1009-3)

Immunogen

Recombinant protein

Host

Mouse

Positive Control

Human placenta tissue lysate, HepG2, human spleen tissue, human colon cancer tissue, human thyroid tissue, SH-SY5Y cell.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

2-F6

PROPERTIES

Form

Liquid

Storage Condition

Store at +4Cafter thawing. Aliquot store at -20Cor -80CAvoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

57 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

C14orf93

SYNONYMS

C14orf93 antibody; CN093_HUMAN antibody; Uncharacterized protein C14orf93 antibody; Uncharacterized protein C14orf93 homolog antibody

SUBCELLULAR LOCATION

Secreted. Extracellular region.

FUNCTION

C14orf93 (also named as Regulator of Thyroid Function and Cancer, RTFC) as a novel susceptibility gene for familial nonmedullary thyroid cancer.The oncogenic functions of R115Q, V205M, and G209D RTFC mutants are demonstrated by cell surviving assay, migration assay, and colony forming assays. Moreover, RTFC has been identified as a potential antigen associated with the pathogenesis of peripheral T-cell lymphomas, not otherwise specified (PTCL, NOS). Two in vitro biochemical screens suggested that RTFC might have RNA and phosphopeptide (pSer/pThr-X-X-X-pSer/pThr) binding activities. Yet, the role of RTFC in normal development, as well as the molecular function of RTFC, remain unexplored.