PRODUCT CODE: M1007-22

C12orf51 Mouse Monoclonal Antibody [A2-F5-F10] (M1007-22)

Applications

  • WB

  • IHC-P

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of C12orf51 on SH-SY5Y (1) and A549 (2) using anti-C12orf51 antibody at 1/100 dilution.
  • Western blot analysis of C12orf51 on SH-SY5Y (1) and A549 (2) using anti-C12orf51 antibody at 1/100 dilution.
  • ICC staining C12orf51 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining C12orf51 (green) in PC-3M cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-C12orf51 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-C12orf51 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-C12orf51 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY5Y cells with C12orf51 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of C12orf51 on SH-SY5Y (1) and A549 (2) using anti-C12orf51 antibody at 1/100 dilution.

Applications

  • WB

  • IHC-P

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

C12orf51 Mouse Monoclonal Antibody [A2-F5-F10] (M1007-22)

Immunogen

Recombinant protein

Host

Mouse

Positive Control

HepG2, A549, SH-SY5Y, PC-3M, human colon cancer tissue, human kidney tissue, mouse testis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A2-F5-F10

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

438/145/98/262 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50 IHC-P

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

C12orf51

SYNONYMS

C12orf51 antibody; HECT domain-containing protein 4 antibody; HECTD4 antibody; KIAA0614 antibody; Probable E3 ubiquitin-protein ligase HECTD4 antibody

SUBCELLULAR LOCATION

Membrane. Single-pass membrane protein.

FUNCTION

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.