PRODUCT CODE: M1012-4

C10orf58 Mouse Monoclonal Antibody [7-B7] (M1012-4)

Applications

  • WB

  • ICC

REACTIVITY

  • Human

Western blot analysis of C10orf58 on recombinant protein using anti-C10orf58 antibody at 1/1000 dilution.
  • Western blot analysis of C10orf58 on recombinant protein using anti-C10orf58 antibody at 1/1000 dilution.
  • Western blot analysis of C10orf58 on human skin tissue lysate using anti-C10orf58 antibody at 1/1000 dilution.
  • ICC staining C10orf58 (green) in HUVEC cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining C10orf58 (green) in PC-3M cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining C10orf58 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of C10orf58 on recombinant protein using anti-C10orf58 antibody at 1/1000 dilution.

Applications

  • WB

  • ICC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

C10orf58 Mouse Monoclonal Antibody [7-B7] (M1012-4)

Immunogen

Recombinant protein

Host

Mouse

Positive Control

Recombinant protein, human skin tissue lysate, HepG2, HUVEC, PC-3M.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

7-B7

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

Isotype

IgG2a

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

C10orf58

SYNONYMS

C10orf58 antibody; CJ058_HUMAN antibody; UPF0765 protein C10orf58 antibody

SEQUENCE SIMILARITIES

Belongs to the peroxiredoxin-like PRXL2 family. PRXL2A subfamily.

TISSUE SPECIFICITY

Expressed in CSF1 and TNFSF11-stimulated CD14(+) peripheral blood mononuclear cells (PBMCs).

SUBCELLULAR LOCATION

Secreted.

FUNCTION

FAM213A, also known as PAMM (peroxiredoxin-like 2 activated in M-CSF stimulated monocytes) or C10orf58, is a 229 amino acid cytoplasmic protein that belongs to the peroxiredoxin-like FAM213 family and FAM213A subfamily. FAM213A plays a role in redox regulation of the cell, acts as an antioxidant, and is induced by CSF1 in peripheral blood mononuclear cells (PBMCs). Existing as two alternatively spliced isoforms, the gene encoding FAM213A maps to human chromosome 10. Spanning nearly 135 million base pairs, chromosome 10 makes up approximately 4.5% of total DNA in cells and encodes nearly 1,200 genes.