c-Myc Recombinant Rabbit Monoclonal Antibody [JE01-20] (HA722895)
Catalog# HA722895
Rabbit Monoclonal to c-Myc
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WB
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IF-Cell
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Human
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Mouse
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Rat
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Monkey
Lane 1: HeLa cell lysate
Lane 2: A549 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: C6 cell lysate
Lane 7: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 55 kDa
Exposure time: 14 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722895) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
c-Myc Recombinant Rabbit Monoclonal Antibody [JE01-20] (HA722895)
Immunogen
Synthetic peptide within human c-myc aa 1-50 / 439.
Host
Rabbit
Positive Control
HeLa cell lysate, A549 cell lysate, HepG2 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, C6 cell lysate, COS-1 cell lysate, HeLa, NIH/3T3.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE01-20
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 50 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
c-Myc
SYNONYMS
AU016757 antibody;Avian myelocytomatosis viral oncogene homolog antibody;bHLHe39 antibody;c Myc antibody;Cellular myelocytomatosis oncogene antibody;Class E basic helix-loop-helix protein 39 antibody;MGC105490 antibody;MRTL antibody;Myc antibody;Myc protein antibody;Myc proto oncogene protein antibody;Myc proto-oncogene protein antibody;myc-related translation/localization regulatory factor antibody;MYC_HUMAN antibody;Myc2 antibody;myca antibody;MYCC antibody;Myelocytomatosis oncogene a antibody;Myelocytomatosis oncogene antibody;Niard antibody;Nird antibody;oncogene c-Myc antibody;Oncogene Myc antibody;OTTHUMP00000158589 antibody;OTTHUMP00000227763 antibody;Proto-oncogene c-Myc antibody;Protooncogene homologous to myelocytomatosis virus antibody;RNCMYC antibody;Transcription factor p64 antibody;Transcriptional regulator Myc-A antibody;V-Myc avian myelocytomatosis viral oncogene homolog antibody;v-myc myelocytomatosis viral oncogene homolog (avian) antibody;zc-myc antibody
SUBCELLULAR LOCATION
Nucleus, nucleoplasm, nucleolus, Cytoplasm.
FUNCTION
Myc proteins are transcription factors that activate expression of many pro-proliferative genes through binding enhancer box sequences (E-boxes) and recruiting histone acetyltransferases (HATs). Myc is thought to function by upregulating transcript elongation of actively transcribed genes through the recruitment of transcriptional elongation factors. It can also act as a transcriptional repressor. By binding Miz-1 transcription factor and displacing the p300 co-activator, it inhibits expression of Miz-1 target genes. In addition, myc has a direct role in the control of DNA replication.This activity could contribute to DNA amplification in cancer cells.
CITATIONS
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Fan Chengcheng,et al
Natural Product-Inspired Discovery of Naphthoquinone-Furo-Piperidine Derivatives as Novel STAT3 Inhibitors for the Treatment of Triple-Negative Breast Cancer
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WB
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Human
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Citation
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