c-Kit Recombinant Rabbit Monoclonal Antibody [ST04-99] (ET1609-60)
Catalog# ET1609-60
Rabbit Monoclonal to c-Kit
-
WB
-
IHC-P
-
Human
Western blot analysis of c-Kit on different lysates with Rabbit anti-c-Kit antibody (ET1609-60) at 1/2,000 dilution.
Lane 1: Saos-2 cell lysate
Lane 2: HL-60 cell lysate (low expression)
Lane 3: Jurkat cell lysate (low expression)
Lysates/proteins at 15 µg/Lane.
Predicted band size: 110 kDa
Observed band size: 110-140 kDa
Exposure time: 2 minutes 18 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-60) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
c-Kit Recombinant Rabbit Monoclonal Antibody [ST04-99] (ET1609-60)
Immunogen
Synthetic peptide within c-terminal human kit.
Host
Rabbit
Positive Control
Human brain tissue lysates, Saos-2 cell lysate, human breast tissue, human gastrointestinal stromal tumor tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
ST04-99
RRID
APPLICATION DILUTION
-
WB
-
1:2,000
-
IHC-P
-
1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 110 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
c-Kit
SYNONYMS
C Kit antibody;c-Kit antibody;c-Kit Ligand antibody;CD117 antibody;Kit antibody;Kit Ligand antibody;KIT oncogene antibody;KIT proto oncogene receptor tyrosine kinase antibody;KIT_HUMAN antibody;Mast cell growth factor receptor antibody;Mast/stem cell growth factor receptor Kit antibody;MGF antibody;p145 c-kit antibody;PBT antibody;Piebald trait protein antibody;Proto oncogene c Kit antibody;Proto oncogene tyrosine protein kinase Kit antibody;Proto-oncogene c-Kit antibody;SCF Receptor antibody;SCFR antibody;soluble KIT variant 1 antibody;Steel Factor Receptor antibody;Stem cell factor receptor antibody;tyrosine protein kinase Kit antibody;Tyrosine-protein kinase Kit antibody;v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog antibody;v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibody;v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
TISSUE SPECIFICITY
Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.
POST-TRANSLATIONAL MODIFICATION
Ubiquitinated by SOCS6. KIT is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to internalization and degradation.; Autophosphorylated on tyrosine residues. KITLG/SCF binding enhances autophosphorylation. Isoform 1 shows low levels of tyrosine phosphorylation in the absence of added KITLG/SCF (in vitro). Kinase activity is down-regulated by phosphorylation on serine residues by protein kinase C family members. Phosphorylation at Tyr-568 is required for interaction with PTPN11/SHP-2, CRK (isoform Crk-II) and members of the SRC tyrosine-protein kinase family. Phosphorylation at Tyr-570 is required for interaction with PTPN6/SHP-1. Phosphorylation at Tyr-703, Tyr-823 and Tyr-936 is important for interaction with GRB2. Phosphorylation at Tyr-721 is important for interaction with PIK3R1. Phosphorylation at Tyr-823 and Tyr-936 is important for interaction with GRB7.
SUBCELLULAR LOCATION
Cell membrane, Cytoplasm.
FUNCTION
The c-Kit proto-oncogene is a member of the receptor tyrosine kinase family and, more specifically, is closely related to the platelet derived growth factor receptor (PDGFR). c-Kit, the normal cellular homolog of the HZ4-feline sarcoma virus transforming gene (v-Kit), encodes a transmembrane receptor. c-Kit regulates a variety of biological responses including chemotaxis, cell proliferation, apoptosis and adhesion. c-Kit is also identical with the product of the W locus in mice and, as such, is integral to the development of mast cells and hematopoiesis. The ligand for the c-Kit receptor (KL) has been identified and is encoded at the murine steel (SI) locus. Kit is the human homolog of the proto-oncogene c-Kit. Mutations in Kit are integral for tumor growth and progression in various cancers.
