PRODUCT CODE: ER1802-76

BubR1 Rabbit Polyclonal Antibody (ER1802-76)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

Western blot analysis of BubR1 on SH-SY-5Y cell lysate using anti-BubR1 antibody at 1/10,000 dilution.
  • Western blot analysis of BubR1 on SH-SY-5Y cell lysate using anti-BubR1 antibody at 1/10,000 dilution.
  • ICC staining BubR1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining BubR1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining BubR1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-BubR1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HL-60 cells with BubR1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of BubR1 on SH-SY-5Y cell lysate using anti-BubR1 antibody at 1/10,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

BubR1 Rabbit Polyclonal Antibody (ER1802-76)

Immunogen

Recombinant protein within human bubr1 aa 310-620.

Host

Rabbit

Positive Control

Hela, SH-SY-5Y, SiHa, human spleen tissue, HL-60.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

119 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:5,000-1:10,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

BubR1

SYNONYMS

Beta homolg of S. cerevisiae BUB 1 antibody; Beta homolg of S. cerevisiae budding uninhibited by benzimidazoles antibody; BUB 1B antibody; BUB1 budding uninhibited by benzimidazoles 1 homolog beta antibody; Bub1A antibody; BUB1B antibody; BUB1B_HUMAN antibody; BUB1beta antibody; BUBR1 antibody; Budding Uninhibited by Benzimidazoles 1 beta antibody; Budding uninhibited by benzimidazoles 1 homolog beta (yeast) antibody; hBUBR1 antibody; MAD3/BUB1 related protein kinase antibody; MAD3/BUB1-related protein kinase antibody; MAD3L antibody; Mitotic checkpoint gene BUB1B antibody; Mitotic checkpoint kinase MAD3L antibody; Mitotic checkpoint serine/threonine protein kinase BUB1 beta antibody; Mitotic checkpoint serine/threonine-protein kinase BUB1 beta antibody; MVA1 antibody; OTTHUMP00000160319 antibody; Protein SSK1 antibody; SSK 1 antibody; SSK1 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily.

TISSUE SPECIFICITY

Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index.

POST-TRANSLATIONAL MODIFICATION

Proteolytically cleaved by caspase-3 in a cell cycle specific manner. The cleavage might be involved in the durability of the cell cycle delay. Caspase-3 cleavage is associated with abrogation of the mitotic checkpoint. The major site of cleavage is at Asp-610.; Acetylation at Lys-250 regulates its degradation and timing in anaphase entry.; Ubiquitinated. Degraded by the proteasome.; Sumoylated with SUMO2 and SUMO3. The sumoylation mediates the association with CENPE at the kinetochore.; Autophosphorylated in vitro. Intramolecular autophosphorylation is stimulated by CENPE. Phosphorylated during mitosis and hyperphosphorylated in mitotically arrested cells. Phosphorylation at Ser-670 and Ser-1043 occurs at kinetochores upon mitotic entry with dephosphorylation at the onset of anaphase.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm.

FUNCTION

Human cells contain two related protein kinases, BUB1 and BUBR1, that appear to have evolved from a single ancestral BUB1 gene. Both kinases are concentrated near the surface of the kinetochore where they monitor kinetochore-microtubule interactions. BUB1 and BUBR1 bind to kinetochores and are postulated to be components of the mitotic checkpoint, which monitors kinetochore activities to determine if chromosomes have achieved alignment at the spindle equator. BUBR1 is essential for normal mitotic progression as it prevents cells from prematurely entering anaphase. BUB3 is a conserved component of the mitotic spindle assembly complex and is also involved with the essential spindle checkpoint pathway that operates during early embryogenesis.