Lane 1: A549
Lane 2: HL-60
Lane 3: A431
Lane 4: Rat colon
Recombinant Rabbit monoclonal primary
Bub3 Recombinant Rabbit Monoclonal Antibody [JG38-81] (ET7108-82)
Recombinant protein corresponding to the n-terminus of human bub3.
A549, HL-60, A431, rat colon tissue lysate, LOVO, rat testis tissue, human thyroid gland tissue, human colon cancer tissue, mouse fallopian tissue, PC-3M.
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A purified.
Aa2-050 antibody; AU019800 antibody; AU021329 antibody; AU043350 antibody; AW146323 antibody; BUB 3 antibody; BUB 3L antibody; BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog antibody; bub3 antibody; BUB3 budding uninhibited by benzimidazoles 3 homolog antibody; BUB3 mitotic checkpoint protein antibody; BUB3_HUMAN antibody; BUB3L antibody; Budding uninhibited by benomyl antibody; Budding uninhibited by benzimidazoles 3 antibody; Budding uninhibited by benzimidazoles 3 homolog (S. cerevisiae) antibody; Budding uninhibited by benzimidazoles 3 homolog (yeast) antibody; Budding uninhibited by benzimidazoles 3 homolog antibody; Budding uninhibited by benzimidazoles 3, S. cerevisiae, homolog of antibody; C78067 antibody; hBUB3 antibody; Mitotic checkpoint component antibody; Mitotic checkpoint protein bub3 antibody; OTTHUMP00000020679 antibody; OTTHUMP00000020680 antibody; WD repeat type I transmembrane protein A72.5 antibody
Belongs to the WD repeat BUB3 family.
Poly-ADP-ribosylated by PARP1.
BUB3 (budding uninhibited by benzimidazoles 3 homolog), also known as BUB3L or hBUB3, is a conserved component of the mitotic spindle assembly complex (MCC). It contains five WD repeat domains and forms cell cycle constitutive complexes with BUB1 and BUBR1. BUB3 is essential for the kinetochore localization of BUB1 and BUBR1. As a component of the MCC, BUB3 is involved in the essential spindle checkpoint pathway that operates during early embryogenesis. The spindle checkpoint pathway functions to postpone the initiation of anaphase until chromosomes are properly attached to the spindle. This acts to ensure accurate chromosome segregation. In addition, BUB3 plays a role in regulating the establishment of correct kinetochore-microtubule attachments. BUB3 is also thought to bind Tctex1L (or DYNLT3), a dynein light chain.