PRODUCT CODE: ET7108-82

Bub3 Recombinant Rabbit Monoclonal Antibody [JG38-81] (ET7108-82)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IP

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Bub3 on different lysates using anti-Bub3 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: A549<br />
        Lane 2: HL-60<br />
 Lane 3: A431  <br />
      Lane 4: Rat colon
  • Western blot analysis of Bub3 on different lysates using anti-Bub3 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: A549<br />
        Lane 2: HL-60<br />
 Lane 3: A431  <br />
      Lane 4: Rat colon
  • ICC staining Bub3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Bub3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Bub3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Bub3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-Bub3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Bub3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse fallopian tissue using anti-Bub3 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of PC-3M cells with Bub3 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).  Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Bub3 on different lysates using anti-Bub3 antibody at 1/500 dilution.
Positive control:
Lane 1: A549
Lane 2: HL-60
Lane 3: A431
Lane 4: Rat colon

Applications

  • WB

  • ICC

  • IF

  • IP

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Bub3 Recombinant Rabbit Monoclonal Antibody [JG38-81] (ET7108-82)

Immunogen

Recombinant protein corresponding to the n-terminus of human bub3.

Host

Rabbit

Positive Control

A549, HL-60, A431, rat colon tissue lysate, LOVO, rat testis tissue, human thyroid gland tissue, human colon cancer tissue, mouse fallopian tissue, PC-3M.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JG38-81

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

37 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • IP

  • 1:10-1:50 FC

TARGET

UNIPROT #

PROTEIN NAME

Bub3

SYNONYMS

Aa2-050 antibody; AU019800 antibody; AU021329 antibody; AU043350 antibody; AW146323 antibody; BUB 3 antibody; BUB 3L antibody; BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog antibody; bub3 antibody; BUB3 budding uninhibited by benzimidazoles 3 homolog antibody; BUB3 mitotic checkpoint protein antibody; BUB3_HUMAN antibody; BUB3L antibody; Budding uninhibited by benomyl antibody; Budding uninhibited by benzimidazoles 3 antibody; Budding uninhibited by benzimidazoles 3 homolog (S. cerevisiae) antibody; Budding uninhibited by benzimidazoles 3 homolog (yeast) antibody; Budding uninhibited by benzimidazoles 3 homolog antibody; Budding uninhibited by benzimidazoles 3, S. cerevisiae, homolog of antibody; C78067 antibody; hBUB3 antibody; Mitotic checkpoint component antibody; Mitotic checkpoint protein bub3 antibody; OTTHUMP00000020679 antibody; OTTHUMP00000020680 antibody; WD repeat type I transmembrane protein A72.5 antibody

SEQUENCE SIMILARITIES

Belongs to the WD repeat BUB3 family.

POST-TRANSLATIONAL MODIFICATION

Poly-ADP-ribosylated by PARP1.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

BUB3 (budding uninhibited by benzimidazoles 3 homolog), also known as BUB3L or hBUB3, is a conserved component of the mitotic spindle assembly complex (MCC). It contains five WD repeat domains and forms cell cycle constitutive complexes with BUB1 and BUBR1. BUB3 is essential for the kinetochore localization of BUB1 and BUBR1. As a component of the MCC, BUB3 is involved in the essential spindle checkpoint pathway that operates during early embryogenesis. The spindle checkpoint pathway functions to postpone the initiation of anaphase until chromosomes are properly attached to the spindle. This acts to ensure accurate chromosome segregation. In addition, BUB3 plays a role in regulating the establishment of correct kinetochore-microtubule attachments. BUB3 is also thought to bind Tctex1L (or DYNLT3), a dynein light chain.