PRODUCT CODE: ET1611-4

BTK Recombinant Rabbit Monoclonal Antibody [SN69-07] (ET1611-4)

  • Recombinant

Applications

  • WB

  • IP

REACTIVITY

  • Human

Western blot analysis of BTK on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-4, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Daudi cell lysate<br />
Lane 2: Raji cell lysate<br />
Lane 3: U937 cell lysate
  • Western blot analysis of BTK on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-4, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Daudi cell lysate<br />
Lane 2: Raji cell lysate<br />
Lane 3: U937 cell lysate
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-BTK antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-4, 1/50)  for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of BTK on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-4, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Daudi cell lysate
Lane 2: Raji cell lysate
Lane 3: U937 cell lysate

Applications

  • WB

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

BTK Recombinant Rabbit Monoclonal Antibody [SN69-07] (ET1611-4)

Immunogen

Synthetic peptide within c-terminal human btk.

Host

Rabbit

Positive Control

Daudi cell lysate, Raji cell lysate, U937 cell lysate, human tonsil tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN69-07

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

76 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

TARGET

UNIPROT #

PROTEIN NAME

BTK

SYNONYMS

Agammaglobulinaemia tyrosine kinase antibody; AGMX 1 antibody; AGMX1 antibody; AT antibody; ATK antibody; B cell progenitor kinase antibody; B-cell progenitor kinase antibody; BPK antibody; Bruton agammaglobulinemia tyrosine kinase antibody; Bruton tyrosine kinase antibody; Bruton’s Tyrosine Kinase antibody; Btk antibody; BTK_HUMAN antibody; dominant-negative kinase-deficient Brutons tyrosine kinase antibody; IMD 1 antibody; IMD1 antibody; MGC126261 antibody; MGC126262 antibody; OTTHUMP00000063593 antibody; PSCTK 1 antibody; PSCTK1 antibody; truncated Bruton agammaglobulinemia tyrosine kinase antibody; Tyrosine protein kinase BTK antibody; Tyrosine-protein kinase BTK antibody; tyrosine-protein kinase BTK isoform (lacking exon 14 antibody; XLA antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.

TISSUE SPECIFICITY

Predominantly expressed in B-lymphocytes.

POST-TRANSLATIONAL MODIFICATION

Following B-cell receptor (BCR) engagement, translocates to the plasma membrane where it gets phosphorylated at Tyr-551 by LYN and SYK. Phosphorylation at Tyr-551 is followed by autophosphorylation of Tyr-223 which may create a docking site for a SH2 containing protein. Phosphorylation at Ser-180 by PRKCB, leads in translocation of BTK back to the cytoplasmic fraction. Phosphorylation at Ser-21 and Ser-115 creates a binding site for PIN1 at these Ser-Pro motifs, and promotes it's recruitment.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus, Cell membrane.

FUNCTION

The Tec family of non-receptor tyrosine kinases is composed of six proteins designated Tec, Emt (also known as Itk or Tsk), Btk (previously known as Atk, BPK or Emb), Bmx, Txk (also known as Rlk) and Dsrc28C. All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini. Four alternatively spliced forms of Tec are found to be expressed broadly in cells of hematopoietic lineage and hepatocytes. The Emt gene product associates with CD28 and becomes activated subsequent to CD28 ligation. Btk is necessary for proper B cell development, and mutations in the gene encoding Btk have been associated with families suffering from X-linked agammaglobulinemia, also referred to as Bruton's disease. The Bmx protein shares a high degree of homology with Btk and seems to be expressed at highest levels in the heart. Txk expression is T cell-specific, while expression of the Drosophila Tec homolog, Dsrc28C, is developmentally regulated.